HERC3 Antibody

• Background :

  HERC3 belongs to the HERC family of ubiquitin ligases, all of which contain a HECT domain and at least 1 RCC1 (MIM 179710) -like domain (RLD) . The 350-amino acid HECT domain is predicted to catalyze the formation of a thioester with ubiquitin before transferring it to a substrate, and the RLD is predicted to act as a guanine nucleotide exchange factor for small G proteins (Hochrainer et al., 2005 [PubMed 15676274]) .

• NCBI Gene ID :

  8916

• Swiss Prot :

  Q15034

• Accession Number :

  Q15034

• Host :

  Rabbit

• Reactivity :

  Human

• Immunogen :

  This HERC3 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 51-77 amino acids from the N-terminal region of human HERC3.

• Clonality :

  Polyclonal

• Conjugation :

  Unconjugated

• Type :

  Primary Antibodies

• Field of Research :

  Cell Cycle

• Purification :

  This antibody is purified through a protein A column, followed by peptide affinity purification.

• Concentration :

  Batch dependent

• Buffer :

  Supplied in PBS with 0.09% (W/V) sodium azide.

• Modification :

  None

• Shipping Conditions :

  Blue Ice

• Storage Conditions :

  Store at 4˚ C for three months and -20˚ C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

• Calculated Molecular Weight :

  117 kDa

• Fragment :

  Rabbit Ig

• Applications Notes :

  For WB starting dilution is: 1:1000

• Symbol :

  HERC3

• NCBI Official Name :

  Probable E3 ubiquitin-protein ligase HERC3

• NCBI Organism :

  Homo sapiens

• Background Reference 01 :

  Rose, J.E., et al. Mol. Med. 16 (7-8), 247-253 (2010) :

• Background Reference 02 :

  Hochrainer, K., et al. Genomics 85 (2) :153-164 (2005)

• Background Reference 03 :

  Cruz, C., et al. FEBS Lett. 488 (1-2), 74-80 (2001) :

• Background Reference 04 :

  Cruz, C., et al. Cytogenet. Cell Genet. 87 (3-4), 263-264 (1999) :

• Other Product Names :

  Probable E3 ubiquitin-protein ligase HERC3, 632-, HECT domain and RCC1-like domain-containing protein 3, HERC3, KIAA0032

• Tested Applications :

  WB

• Protein ID :

  2495699

• Physical Properties :

  Liquid