Mouse Cdkn2a Antibody

• Background:

  Capable of inducing cell cycle arrest in G1 and G2 phases. Acts as a tumor suppressor. Binds to MDM2 and blocks its nucleocytoplasmic shuttling by sequestering it in the nucleolus. This inhibits the oncogenic action of MDM2 by blocking MDM2-induced degradation of p53 and enhancing p53-dependent transactivation and apoptosis. Also induces G2 arrest and apoptosis in a p53-independent manner by preventing the activation of cyclin B1/CDC2 complexes. Binds to BCL6 and down-regulates BCL6-induced transcriptional repression. Binds to E2F1 and MYC and blocks their transcriptional activator activity but has no effect on MYC transcriptional repression. Binds to TOP1/TOPOI and stimulates its activity. This complex binds to rRNA gene promoters and may play a role in rRNA transcription and/or maturation. Interacts with NPM1/B23 and promotes its polyubiquitination and degradation, thus inhibiting rRNA processing. Interacts with UBE2I/UBC9 and enhances sumoylation of a number of its binding partners including MDM2 and E2F1. Binds to HUWE1 and represses its ubiquitin ligase activity. May play a role in controlling cell proliferation and apoptosis during mammary gland development.

• NCBI Gene ID:

  12578

• Swiss Prot:

  Q64364

• Accession Number:

  Q64364

• Host:

  Rabbit

• Reactivity:

  Mouse

• Immunogen:

  This Mouse Cdkn2a antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 39-65 amino acids from the Central region of mouse Cdkn2a.

• Clonality:

  Polyclonal

• Conjugation:

  Unconjugated

• Type:

  Primary Antibodies

• Field of Research:

  Other

• Purification:

  This antibody is purified through a protein A column, followed by peptide affinity purification.

• Concentration:

  Batch dependent

• Buffer:

  Supplied in PBS with 0.09% (W/V) sodium azide.

• Modification:

  None

• Shipping Conditions:

  Blue Ice

• Storage Conditions:

  Store at 4˚ C for three months and -20˚ C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

• Calculated Molecular Weight:

  19 kDa

• Fragment:

  Rabbit Ig

• Applications Notes:

  For WB starting dilution is: 1:1000

• Symbol:

  Cdkn2a

• NCBI Official Name:

  Cyclin-dependent kinase inhibitor 2A, isoform 3

• NCBI Organism:

  Mus musculus

• Background Reference 01:

  Nogueira, C., et al. Oncogene 29 (47) :6222-6232 (2010)

• Background Reference 02:

  Fernandez-Diaz, L.C., et al. Development 137 (20) :3393-3403 (2010)

• Background Reference 03:

  Bennecke, M., et al. Cancer Cell 18 (2) :135-146 (2010)

• Background Reference 04:

  Negishi, M., et al. PLoS ONE (8), E12373 (2010) :

• Other Product Names:

  Cyclin-dependent kinase inhibitor 2A, isoform 3, p19ARF, Cdkn2a {ECO:0000312|EMBL:AAB357701, ECO:0000312|MGI:MGI:104738}

• Tested Applications:

  WB

• Physical Properties:

  Liquid