NFKB Antibody

• Background:

  This gene encodes a 105 kD protein which can undergo cotranslational processing by the 26S proteasome to produce a 50 kD protein. The 105 kD protein is a Rel protein-specific transcription inhibitor and the 50 kD protein is a DNA binding subunit of the NF-kappa-B (NFKB) protein complex. NFKB is a transcription regulator that is activated by various intra- and extra-cellular stimuli such as cytokines, oxidant-free radicals, ultraviolet irradiation, and bacterial or viral products. Activated NFKB translocates into the nucleus and stimulates the expression of genes involved in a wide variety of biological functions. Inappropriate activation of NFKB has been associated with a number of inflammatory diseases while persistent inhibition of NFKB leads to inappropriate immune cell development or delayed cell growth. Two transcript variants encoding different isoforms have been found for this gene.

• NCBI Gene ID:

  4790

• Swiss Prot:

  P19838

• Accession Number:

  P19838

• Host:

  Rabbit

• Reactivity:

  Human

• Immunogen:

  This NFKB (p105) antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 911-937 amino acids from the C-terminal region of human NFKB (p105) .

• Clonality:

  Polyclonal

• Conjugation:

  Unconjugated

• Type:

  Primary Antibodies

• Field of Research:

  Cancer, Cell Cycle, Immunology, Signal Transduction, Inflammasomes

• Purification:

  This antibody is purified through a protein A column, followed by peptide affinity purification.

• Concentration:

  Batch dependent

• Homology:

  Predicted species reactivity based on immunogen sequence: Chicken, Mouse, Rat

• Buffer:

  Supplied in PBS with 0.09% (W/V) sodium azide.

• Modification:

  None

• Shipping Conditions:

  Blue Ice

• Storage Conditions:

  Store at 4˚ C for three months and -20˚ C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

• Calculated Molecular Weight:

  105 kDa

• Fragment:

  Rabbit Ig

• Applications Notes:

  For WB starting dilution is: 1:1000

• Symbol:

  NFKB1

• NCBI Official Name:

  Nuclear factor NF-kappa-B p105 subunit

• NCBI Organism:

  Homo sapiens

• Background Reference 01:

  Beshir, A.B., et al. Cancer Lett. 299 (2) :137-149 (2010)

• Background Reference 02:

  Song, C., et al. Virology 407 (2) :268-280 (2010)

• Background Reference 03:

  Gonsalves, C., et al. J. Immunol. 185 (10) :6253-6264 (2010)

• Background Reference 04:

  Kingeter, L.M., et al. J. Immunol. 185 (8) :4520-4524 (2010)

• Other Product Names:

  Nuclear factor NF-kappa-B p105 subunit, DNA-binding factor KBF1, EBP-1, Nuclear factor of kappa light polypeptide gene enhancer in B-cells 1, Nuclear factor NF-kappa-B p50 subunit, NFKB1

• Tested Applications:

  WB

• Protein ID:

  21542418

• Physical Properties:

  Liquid