PCBP1 Antibody

• Background:

  This intronless gene is thought to have been generated by retrotransposition of a fully processed PCBP-2 mRNA. This gene and PCBP-2 have paralogues (PCBP3 and PCBP4) which are thought to have arisen as a result of duplication events of entire genes. The protein encoded by this gene appears to be multifunctional. It along with PCBP-2 and hnRNPK corresponds to the major cellular poly (rC) -binding protein. It contains three K-homologous (KH) domains which may be involved in RNA binding. This encoded protein together with PCBP-2 also functions as translational coactivators of poliovirus RNA via a sequence-specific interaction with stem-loop IV of the IRES and promote poliovirus RNA replication by binding to its 5'-terminal cloverleaf structure. It has also been implicated in translational control of the 15-lipoxygenase mRNA, human Papillomavirus type 16 L2 mRNA, and hepatitis A virus RNA. The encoded protein is also suggested to play a part in formation of a sequence-specific alpha-globin mRNP complex which is associated with alpha-globin mRNA stability.

• NCBI Gene ID:

  5093

• Swiss Prot:

  Q15365

• Accession Number:

  Q15365

• Host:

  Rabbit

• Reactivity:

  Human, Mouse

• Immunogen:

  This PCBP1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 188-217 amino acids from the Central region of human PCBP1.

• Clonality:

  Polyclonal

• Conjugation:

  Unconjugated

• Type:

  Primary Antibodies

• Field of Research:

  Other

• Purification:

  This antibody is purified through a protein A column, followed by peptide affinity purification.

• Concentration:

  Batch dependent

• Homology:

  Predicted species reactivity based on immunogen sequence: Bovine, Rabbit

• Buffer:

  Supplied in PBS with 0.09% (W/V) sodium azide.

• Modification:

  None

• Shipping Conditions:

  Blue Ice

• Storage Conditions:

  Store at 4˚ C for three months and -20˚ C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

• Calculated Molecular Weight:

  37 kDa

• Fragment:

  Rabbit Ig

• Applications Notes:

  For WB starting dilution is: 1:1000

• Symbol:

  PCBP1

• NCBI Official Name:

  Poly (rC) -binding protein 1

• NCBI Organism:

  Homo sapiens

• Background Reference 01:

  Cloke, B., et al. Endocrinology 151 (8) :3954-3964 (2010)

• Background Reference 02:

  Wang, H., et al. Cancer Cell 18 (1) :52-62 (2010)

• Background Reference 03:

  Zhang, T., et al. Mol. Cancer 9, 72 (2010) :

• Background Reference 04:

  Waggoner, S.A., et al. J. Biol. Chem. 284 (14) :9039-9049 (2009)

• Other Product Names:

  Poly (rC) -binding protein 1, Alpha-CP1, Heterogeneous nuclear ribonucleoprotein E1, hnRNP E1, Nucleic acid-binding protein SUB23, PCBP1

• Tested Applications:

  WB

• Protein ID:

  42560548

• Physical Properties:

  Liquid