SNAP25 Antibody

• Background:

  Synaptic vesicle membrane docking and fusion is mediated by SNAREs (soluble N-ethylmaleimide-sensitive factor attachment protein receptors) located on the vesicle membrane (v-SNAREs) and the target membrane (t-SNAREs) . The assembled v-SNARE/t-SNARE complex consists of a bundle of four helices, one of which is supplied by v-SNARE and the other three by t-SNARE. For t-SNAREs on the plasma membrane, the protein syntaxin supplies one helix and the protein encoded by this gene contributes the other two. Therefore, this gene product is a presynaptic plasma membrane protein involved in the regulation of neurotransmitter release. Two alternative transcript variants encoding different protein isoforms have been described for this gene.

• NCBI Gene ID:

  6616

• Swiss Prot:

  P60880

• Accession Number:

  P60880

• Host:

  Rabbit

• Reactivity:

  Human

• Immunogen:

  This SNAP25 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 36-63 amino acids from the Central region of human SNAP25.

• Clonality:

  Polyclonal

• Conjugation:

  Unconjugated

• Type:

  Primary Antibodies

• Field of Research:

  Cancer, Obesity, Neuroscience, Signal Transduction

• Purification:

  This antibody is purified through a protein A column, followed by peptide affinity purification.

• Concentration:

  Batch dependent

• Homology:

  Predicted species reactivity based on immunogen sequence: Zebrafish, Bovine, Chicken, Mouse, Rat

• Buffer:

  Supplied in PBS with 0.09% (W/V) sodium azide.

• Modification:

  None

• Shipping Conditions:

  Blue Ice

• Storage Conditions:

  Store at 4˚ C for three months and -20˚ C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

• Calculated Molecular Weight:

  23 kDa

• Fragment:

  Rabbit Ig

• Applications Notes:

  For WB starting dilution is: 1:1000

• Symbol:

  SNAP25

• NCBI Official Name:

  Synaptosomal-associated protein 25

• NCBI Organism:

  Homo sapiens

• Background Reference 01:

  Shimada, M., et al. Hum. Genet. 128 (4) :433-441 (2010)

• Background Reference 02:

  Greaves, J., et al. J. Biol. Chem. 285 (32) :24629-24638 (2010)

• Background Reference 03:

  Oner, O., et al.Atten Disord (2010) In press :

• Background Reference 04:

  Zhang, H., et al. Eur. J. Paediatr. Neurol. (2010) In press :

• Other Product Names:

  Synaptosomal-associated protein 25, SNAP-25, Super protein, SUP, Synaptosomal-associated 25 kDa protein, SNAP25, SNAP

• Tested Applications:

  WB

• Protein ID:

  46397726

• Physical Properties:

  Liquid