POLR3C Antibody

• Background:

  DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates. Specific core component of RNA polymerase III which synthesizes small RNAs, such as 5S rRNA and tRNAs. May direct with other members of the subcomplex RNA Pol III binding to the TFIIIB-DNA complex via the interactions between TFIIIB and POLR3F. May be involved either in the recruitment and stabilization of the subcomplex within RNA polymerase III, or in stimulating catalytic functions of other subunits during initiation. Plays a key role in sensing and limiting infection by intracellular bacteria and DNA viruses. Acts as nuclear and cytosolic DNA sensor involved in innate immune response. Can sense non-self dsDNA that serves as template for transcription into dsRNA. The non-self RNA polymerase III transcripts, such as Epstein-Barr virus-encoded RNAs (EBERs) induce type I interferon and NF-Kappa-B through the RIG-I pathway.

• NCBI Gene ID:

  10623

• Swiss Prot:

  Q9BUI4

• Accession Number:

  Q9BUI4

• Host:

  Rabbit

• Reactivity:

  Human

• Immunogen:

  This POLR3C antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 202-230 amino acids from the Central region of human POLR3C.

• Clonality:

  Polyclonal

• Conjugation:

  Unconjugated

• Type:

  Primary Antibodies

• Field of Research:

  Other

• Purification:

  This antibody is purified through a protein A column, followed by peptide affinity purification.

• Concentration:

  Batch dependent

• Buffer:

  Supplied in PBS with 0.09% (W/V) sodium azide.

• Modification:

  None

• Shipping Conditions:

  Blue Ice

• Storage Conditions:

  Store at 4˚ C for three months and -20˚ C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

• Calculated Molecular Weight:

  61 kDa

• Fragment:

  Rabbit Ig

• Applications Notes:

  For WB starting dilution is: 1:1000

• Symbol:

  POLR3C

• NCBI Official Name:

  DNA-directed RNA polymerase III subunit RPC3

• NCBI Organism:

  Homo sapiens

• Background Reference 01:

  Ablasser, A., et al. Nat. Immunol. 10 (10) :1065-1072 (2009)

• Background Reference 02:

  Hu, P., et al. Mol. Cell. Biol. 22 (22) :8044-8055 (2002)

• Background Reference 03:

  Kuwana, M., et al. Arthritis Rheum. 46 (10) :2742-2747 (2002)

• Background Reference 04:

  Hsieh, Y.J., et al. Mol. Cell. Biol. 19 (11) :7697-7704 (1999)

• Other Product Names:

  DNA-directed RNA polymerase III subunit RPC3, RNA polymerase III subunit C3, DNA-directed RNA polymerase III subunit C, RNA polymerase III 62 kDa subunit, RPC62, POLR3C

• Tested Applications:

  WB

• Protein ID:

  60393871

• Physical Properties:

  Liquid