USP7 Antibody

• Background:

  Hydrolase that deubiquitinates target proteins such as FOXO4, TP53, MDM2, PTEN and DAXX. Together with DAXX, prevents MDM2 self-ubiquitination and enhances the E3 ligase activity of MDM2 towards TP53, thereby promoting TP53 ubiquitination and proteasomal degradation. Deubiquitinates TP53 and MDM2 and strongly stabilizes TP53 even in the presence of excess MDM2, and also induces TP53-dependent cell growth repression and apoptosis. Deubiquitination of FOXO4 in presence of hydrogen peroxide is not dependent on TP53 and inhibits FOXO4-induced transcriptional activity. In association with DAXX, is involved in the deubiquitination and translocation of PTEN from the nucleus to the cytoplasm, both processes that are counteracted by PML. Involved in cell proliferation during early embryonic development. Contributes to the overall stabilization and trans-activation capability of the herpesvirus 1 trans-acting transcriptional protein ICP0/VMW110 during HSV-1 infection.

• NCBI Gene ID:

  7874

• Swiss Prot:

  Q93009

• Accession Number:

  Q93009

• Host:

  Rabbit

• Reactivity:

  Human

• Immunogen:

  This USP7 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 320-348 amino acids from the Central region of human USP7.

• Clonality:

  Polyclonal

• Conjugation:

  Unconjugated

• Type:

  Primary Antibodies

• Field of Research:

  Cancer, Cell Cycle

• Purification:

  This antibody is purified through a protein A column, followed by peptide affinity purification.

• Concentration:

  Batch dependent

• Homology:

  Predicted species reactivity based on immunogen sequence: Rat

• Buffer:

  Supplied in PBS with 0.09% (W/V) sodium azide.

• Modification:

  None

• Shipping Conditions:

  Blue Ice

• Storage Conditions:

  Store at 4˚ C for three months and -20˚ C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

• Calculated Molecular Weight:

  128 kDa

• Fragment:

  Rabbit Ig

• Applications Notes:

  For WB starting dilution is: 1:1000

• Symbol:

  USP7

• NCBI Official Name:

  Ubiquitin carboxyl-terminal hydrolase 7

• NCBI Organism:

  Homo sapiens

• Background Reference 01:

  Sarkari, F., et al. J. Mol. Biol. 402 (5) :825-837 (2010)

• Background Reference 02:

  De Bie, P., et al. Biochem. Biophys. Res. Commun. 400 (3) :389-395 (2010)

• Background Reference 03:

  Maertens, G.N., et al. EMBO J. 29 (15) :2553-2565 (2010)

• Background Reference 04:

  Rose, J.E., et al. Mol. Med. 16 (7-8), 247-253 (2010) :

• Other Product Names:

  Ubiquitin carboxyl-terminal hydrolase 7, Deubiquitinating enzyme 7, Herpesvirus-associated ubiquitin-specific protease, Ubiquitin thioesterase 7, Ubiquitin-specific-processing protease 7, USP7, HAUSP

• Tested Applications:

  WB

• Protein ID:

  212276477

• Physical Properties:

  Liquid