GPC3 Antibody

• Background:

  Cell surface heparan sulfate proteoglycans are composed of a membrane-associated protein core substituted with a variable number of heparan sulfate chains. Members of the glypican-related integral membrane proteoglycan family (GRIPS) contain a core protein anchored to the cytoplasmic membrane via a glycosyl phosphatidylinositol linkage. These proteins may play a role in the control of cell division and growth regulation. The protein encoded by this gene can bind to and inhibit the dipeptidyl peptidase activity of CD26, and it can induce apoptosis in certain cell types. Deletion mutations in this gene are associated with Simpson-Golabi-Behmel syndrome, also known as Simpson dysmorphia syndrome. Alternative splicing results in multiple transcript variants.

• NCBI Gene ID:

  2719

• Swiss Prot:

  P51654

• Accession Number:

  P51654

• Host:

  Rabbit

• Reactivity:

  Human

• Immunogen:

  This GPC3 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 465-494 amino acids from the C-terminal region of human GPC3.

• Clonality:

  Polyclonal

• Conjugation:

  Unconjugated

• Type:

  Primary Antibodies

• Field of Research:

  Cancer, Cell Cycle, Signal Transduction

• Purification:

  This antibody is purified through a protein A column, followed by peptide affinity purification.

• Concentration:

  Batch dependent

• Buffer:

  Supplied in PBS with 0.09% (W/V) sodium azide.

• Modification:

  None

• Shipping Conditions:

  Blue Ice

• Storage Conditions:

  Store at 4˚ C for three months and -20˚ C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

• Calculated Molecular Weight:

  66 kDa

• Fragment:

  Rabbit Ig

• Applications Notes:

  For WB starting dilution is: 1:1000

• Symbol:

  GPC3

• NCBI Official Name:

  Glypican-3

• NCBI Organism:

  Homo sapiens

• Background Reference 01:

  Lai, J.P., et al. Hepatology 52 (5) :1680-1689 (2010)

• Background Reference 02:

  Sakurai, M., et al. Gynecol. Oncol. 119 (2) :332-336 (2010)

• Background Reference 03:

  Zynger, D.L., et al. Histopathology 56 (6) :750-757 (2010)

• Background Reference 04:

  Davoodi, J., et al. Proteomics 7 (13) :2300-2310 (2007)

• Other Product Names:

  Glypican-3, GTR2-2, Intestinal protein OCI-5, MXR7, Secreted glypican-3, GPC3, OCI5

• Tested Applications:

  WB

• Protein ID:

  1708022

• Physical Properties:

  Liquid