Mouse Akt1 Antibody

• Background:

  This gene encodes the founding member of the Akt serine-threonine protein kinase gene family that also includes Akt2 and Akt3. This kinase is a major downstream effector of the phosphatidylinositol 3-kinase (PI3K) pathway that mediates the effects of various growth factors such as platelet-derived growth factor (PDGF), epidermal growth factor (EGF), insulin and insulin-like growth factor I (IGF-I) . It is activated through recruitment to cellular membranes by PI3K lipid products and by phosphorylation by 3-phosphoinositide dependent kinase-1. It then further phosphorylates different downstream proteins in response to various extracellular signals and thus plays a pivotal role in mediating a variety of cellular processes, such as glucose metabolism, glycogen biosynthesis, protein synthesis and turn over, inflammatory response, cell survival (anti-apoptosis) and development. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq].

• NCBI Gene ID:

  11651

• Swiss Prot:

  P31750

• Accession Number:

  P31750

• Host:

  Rabbit

• Reactivity:

  Mouse

• Immunogen:

  This Mouse Akt1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 22-49 amino acids from the N-terminal region of mouse Akt1.

• Clonality:

  Polyclonal

• Conjugation:

  Unconjugated

• Type:

  Primary Antibodies

• Field of Research:

  Cancer, Cell Cycle, Neuroscience, Signal Transduction

• Purification:

  This antibody is purified through a protein A column, followed by peptide affinity purification.

• Concentration:

  Batch dependent

• Homology:

  Predicted species reactivity based on immunogen sequence: Human

• Buffer:

  Supplied in PBS with 0.09% (W/V) sodium azide.

• Modification:

  None

• Shipping Conditions:

  Blue Ice

• Storage Conditions:

  Store at 4˚ C for three months and -20˚ C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

• Calculated Molecular Weight:

  56 kDa

• Fragment:

  Rabbit Ig

• Symbol:

  Akt1

• NCBI Official Name:

  RAC-alpha serine/threonine-protein kinase

• NCBI Organism:

  Mus musculus

• Background Reference 01:

  Chang, Z., et al. Dev. Biol. 347 (2) :384-391 (2010)

• Background Reference 02:

  Zhou, F., et al. Am. J. Pathol. 177 (4) :2124-2133 (2010)

• Background Reference 03:

  Sun, X., et al. Proc. Natl. Acad. Sci. U.S.A. 107 (39) :16887-16892 (2010)

• Background Reference 04:

  Goncalves, M.D., et al. PLoS ONE (9), E12707 (2010) :

• Other Product Names:

  RAC-alpha serine/threonine-protein kinase, AKT1 kinase, Protein kinase B, PKB, Protein kinase B alpha, PKB alpha, Proto-oncogene c-Akt, RAC-PK-alpha, Thymoma viral proto-oncogene, Akt1, Akt, Rac

• Tested Applications:

  WB, IHC-P

• Physical Properties:

  Liquid