PSMD10 Antibody

• Background:

  The 26S proteasome is a multicatalytic proteinase complex with a highly ordered structure composed of 2 complexes, a 20S core and a 19S regulator. The 20S core is composed of 4 rings of 28 non-identical subunits; 2 rings are composed of 7 alpha subunits and 2 rings are composed of 7 beta subunits. The 19S regulator is composed of a base, which contains 6 ATPase subunits and 2 non-ATPase subunits, and a lid, which contains up to 10 non-ATPase subunits. Proteasomes are distributed throughout eukaryotic cells at a high concentration and cleave peptides in an ATP/ubiquitin-dependent process in a non-lysosomal pathway. An essential function of a modified proteasome, the immunoproteasome, is the processing of class I MHC peptides. This gene encodes a non-ATPase subunit of the 19S regulator. Two transcripts encoding different isoforms have been described. Pseudogenes have been identified on chromosomes 3 and 20.

• NCBI Gene ID:

  5716

• Swiss Prot:

  O75832

• Accession Number:

  O75832

• Host:

  Rabbit

• Reactivity:

  Human

• Immunogen:

  This PSMD10 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 184-213 amino acids from the C-terminal region of human PSMD10.

• Clonality:

  Polyclonal

• Conjugation:

  Unconjugated

• Type:

  Primary Antibodies

• Field of Research:

  Cancer, Cell Cycle

• Purification:

  This antibody is purified through a protein A column, followed by peptide affinity purification.

• Concentration:

  Batch dependent

• Homology:

  Predicted species reactivity based on immunogen sequence: Mouse, Rat

• Buffer:

  Supplied in PBS with 0.09% (W/V) sodium azide.

• Modification:

  None

• Shipping Conditions:

  Blue Ice

• Storage Conditions:

  Store at 4˚ C for three months and -20˚ C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

• Calculated Molecular Weight:

  24 kDa

• Fragment:

  Rabbit Ig

• Applications Notes:

  For WB starting dilution is: 1:1000

• Symbol:

  PSMD10

• NCBI Official Name:

  26S proteasome non-ATPase regulatory subunit 10

• NCBI Organism:

  Homo sapiens

• Background Reference 01:

  Meng, Y., et al. Cancer Lett. 297 (1) :9-17 (2010)

• Background Reference 02:

  Man, J.H., et al. J. Clin. Invest. 120 (8) :2829-2841 (2010)

• Background Reference 03:

  Piton, A., et al. Mol. Psychiatry (2010) In press :

• Background Reference 04:

  Serquera, D., et al. Biophys. J. 98 (7) :1294-1301 (2010)

• Other Product Names:

  26S proteasome non-ATPase regulatory subunit 10, 26S proteasome regulatory subunit p28, Gankyrin, p28 (GANK), PSMD10

• Tested Applications:

  WB

• Physical Properties:

  Liquid