ARNT Antibody

• Background:

  The aryl hydrocarbon (Ah) receptor is involved in the induction of several enzymes that participate in xenobiotic metabolism. The ligand-free, cytosolic form of the Ah receptor is complexed to heat shock protein 90. Binding of ligand, which includes dioxin and polycyclic aromatic hydrocarbons, results in translocation of the ligand-binding subunit only to the nucleus. Induction of enzymes involved in xenobiotic metabolism occurs through binding of the ligand-bound Ah receptor to xenobiotic responsive elements in the promoters of genes for these enzymes. This gene encodes a protein that forms a complex with the ligand-bound Ah receptor, and is required for receptor function. The encoded protein has also been identified as the beta subunit of a heterodimeric transcription factor, hypoxia-inducible factor 1. A t (1;12) (q21; p13) translocation, which results in a TEL-ARNT fusion protein, is associated with acute myeloblastic leukemia. Alternative splicing results in multiple transcript variants.

• NCBI Gene ID:

  405

• Swiss Prot:

  P27540

• Accession Number:

  P27540

• Host:

  Rabbit

• Reactivity:

  Human

• Immunogen:

  This ARNT antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 513-544 amino acids from the Central region of human ARNT.

• Clonality:

  Polyclonal

• Conjugation:

  Unconjugated

• Type:

  Primary Antibodies

• Field of Research:

  Cancer, Obesity, Signal Transduction

• Purification:

  This antibody is purified through a protein G column, followed by dialysis against PBS.

• Concentration:

  Batch dependent

• Homology:

  Predicted species reactivity based on immunogen sequence: Bovine

• Buffer:

  Supplied in PBS with 0.09% (W/V) sodium azide.

• Modification:

  None

• Shipping Conditions:

  Blue Ice

• Storage Conditions:

  Store at 4˚ C for three months and -20˚ C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

• Calculated Molecular Weight:

  87 kDa

• Fragment:

  Rabbit Ig

• Symbol:

  ARNT

• NCBI Official Name:

  Aryl hydrocarbon receptor nuclear translocator

• NCBI Organism:

  Homo sapiens

• Background Reference 01:

  Otsubo, K., et al. Cancer Genet. Cytogenet. 202 (1) :22-26 (2010)

• Background Reference 02:

  Bailey, S.D., et al. Diabetes Care 33 (10) :2250-2253 (2010)

• Background Reference 03:

  Jugessur, A., et al. PLoS ONE (7), E11493 (2010) :

• Background Reference 04:

  Kewley, R.J., et al. Biochem. Biophys. Res. Commun. 338 (1) :660-667 (2005)

• Other Product Names:

  Aryl hydrocarbon receptor nuclear translocator, ARNT protein, Class E basic helix-loop-helix protein 2, bHLHe2, Dioxin receptor, nuclear translocator, Hypoxia-inducible factor 1-beta, HIF-1-beta, HIF1-beta, ARNT, BHLHE2

• Tested Applications:

  WB, IF

• Protein ID:

  114163

• Physical Properties:

  Liquid