CBX5 Antibody

• Background:

  This gene encodes a highly conserved nonhistone protein, which is a member of the heterochromatin protein family. The protein is enriched in the heterochromatin and associated with centromeres. The protein has a single N-terminal chromodomain which can bind to histone proteins via methylated lysine residues, and a C-terminal chromo shadow-domain (CSD) which is responsible for the homodimerization and interaction with a number of chromatin-associated nonhistone proteins. The encoded product is involved in the formation of functional kinetochore through interaction with essential kinetochore proteins. The gene has a pseudogene located on chromosome 3. Multiple alternatively spliced variants, encoding the same protein, have been identified.

• NCBI Gene ID:

  23468

• Swiss Prot:

  P45973

• Accession Number:

  P45973

• Host:

  Rabbit

• Reactivity:

  Human

• Immunogen:

  This CBX5 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 88-117 amino acids from the Central region of human CBX5.

• Clonality:

  Polyclonal

• Conjugation:

  Unconjugated

• Type:

  Primary Antibodies

• Field of Research:

  Other

• Purification:

  This antibody is purified through a protein A column, followed by peptide affinity purification.

• Concentration:

  Batch dependent

• Homology:

  Predicted species reactivity based on immunogen sequence: Mouse

• Buffer:

  Supplied in PBS with 0.09% (W/V) sodium azide.

• Modification:

  None

• Shipping Conditions:

  Blue Ice

• Storage Conditions:

  Store at 4˚ C for three months and -20˚ C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

• Calculated Molecular Weight:

  22 kDa

• Fragment:

  Rabbit Ig

• Applications Notes:

  For WB starting dilution is: 1:1000

• Symbol:

  CBX5

• NCBI Official Name:

  Chromobox protein homolog 5

• NCBI Organism:

  Homo sapiens

• Background Reference 01:

  Nozawa, R.S., et al. Nat. Cell Biol. 12 (7) :719-727 (2010)

• Background Reference 02:

  Zeng, W., et al. Epigenetics 5 (4) :287-292 (2010)

• Background Reference 03:

  Emelyanov, A.V., et al. J. Biol. Chem. 285 (20) :15027-15037 (2010)

• Background Reference 04:

  Kiyomitsu, T., et al. J. Cell Biol. 188 (6) :791-807 (2010)

• Other Product Names:

  Chromobox protein homolog 5, Antigen p25, Heterochromatin protein 1 homolog alpha, HP1 alpha, CBX5, HP1A

• Tested Applications:

  WB

• Protein ID:

  1170338

• Physical Properties:

  Liquid