HtrA3 Antibody

• Background:

  Insulin-like growth factors (IGFs) stimulate the proliferation and differentiation of a vast number of cell types. The action of the growth factors is mediated and controlled by a complex system of components, including several proteases that cleave the IGF-Binding Proteins. HtrA1 is a 480 aa protein that contains an N-terminus homologous to MAC25 (IGFBP7) with a conserved Kazal-type serine protease inhibitor motif, as well as a C-terminal PDZ domain. Semiquantitative RT-PCR and immunoblot analyses showed an approximately 7-fold increase of PRSS11 in osteoarthritis cartilage compared with controls. HTRA2 is released from mitochondria and inhibits the function of XIAP by direct binding in a way similar to SMAC. Moreover, when overexpressed extramitochondrially, HTRA2 induced atypical cell death, which was neither accompanied by a significant increase in caspase activity nor inhibited by caspase inhibitors, including XIAP. A catalytically inactive mutant of HTRA2, however, did not induce cell death. Suzuki et al. (2001) concluded that HTRA2 is a SMAC-like inhibitor of IAP (inhibitor of apoptosis proteins) activity with a serine protease-dependent cell death-inducing activity.

• NCBI Gene ID:

  94031

• Swiss Prot:

  P83110

• Accession Number:

  P83110

• Host:

  Rabbit

• Reactivity:

  Human, Rat

• Immunogen:

  This HtrA3 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 112-144 amino acids from the N-terminal region of human HtrA3.

• Clonality:

  Polyclonal

• Conjugation:

  Unconjugated

• Type:

  Primary Antibodies

• Field of Research:

  Signal Transduction

• Purification:

  This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis

• Concentration:

  Batch dependent

• Buffer:

  Supplied in PBS with 0.09% (W/V) sodium azide.

• Modification:

  None

• Shipping Conditions:

  Blue Ice

• Storage Conditions:

  Store at 4˚ C for three months and -20˚ C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

• Calculated Molecular Weight:

  49 kDa

• Fragment:

  Rabbit Ig

• Symbol:

  HTRA3

• NCBI Official Name:

  Serine protease HTRA3

• NCBI Organism:

  Homo sapiens

• Background Reference 01:

  Nie, G.Y., et al., Biochem. J. 371 (Pt 1), 39-48 (2003) .

• Other Product Names:

  Serine protease HTRA3, 3421-, High-temperature requirement factor A3, Pregnancy-related serine protease, HTRA3, PRSP

• Tested Applications:

  WB, IHC-P

• Protein ID:

  21542412

• Physical Properties:

  Liquid