Parkin (phospho Ser101) Antibody

• Background:

  Parkin is an E3 ligase in the ubiquitin-proteasome system. Hereditary Parkinson’s disease is most commonly caused by mutations in the parkin gene and is characterized by the progressive loss of dopaminergic neurons and the presence of Lewy bodies in the substania nigra (Jenner aet al.,1992) . Recent evidence suggests that phosphorylation of parkin at Ser101 may have an important regulatory role on its E3 ubiquitin ligase activity (Yamamoto et al., 2005) .

• NCBI Gene ID:

  5071

• Swiss Prot:

  O60260

• Accession Number:

  O60260

• Host:

  Rabbit

• Reactivity:

  Human, Bovine

• Immunogen:

  Phosphopeptide corresponding to amino acid residues surrounding the phospho-Ser101 of human parkin.

• Clonality:

  Polyclonal

• Conjugation:

  Unconjugated

• Type:

  Primary Antibodies

• Field of Research:

  Phospho-Specific

• Purification:

  Affinity Purified

• Concentration:

  Batch dependent

• Modification:

  Phospho-Specific

• Shipping Conditions:

  Blue Ice

• Storage Conditions:

  Parkin antibody can be stored at -20˚ C and is stable at -20˚ C for at least 1 year.

• Calculated Molecular Weight:

  52

• Applications Notes:

  The antibody has been directly tested for reactivity in Western blots with human tissue. It is anticipated that the antibody will react with bovine and non-human primate tissues based on the fact that these species have 100% homology with the amino acid sequence used as antigen.

• Symbol:

  PARK2

• NCBI Official Name:

  Parkin RBR E3 ubiquitin protein ligase

• NCBI Organism:

  Homo sapiens

• Background Reference 01:

  Jenner P, Dexter DT, Sian J, Schapira AH, Marsden CD (1992) Oxidative stress as a cause of nigral cell death in Parkinson’s disease and incidental Lewy body disease. Ann Neurol. 32 Suppl: S82-7.

• Background Reference 02:

  Yamamoto A, Friedlein A, Imai Y, Takahashi R, Kahle PJ, Haass C (2005) Parkin phosphorylation and modulation of its E# ubiquitin ligase activity. J Biol chem. 280 (5) :3390-9

• Tested Applications:

  WB

• Protein ID:

  116242725

• Physical Properties:

  Liquid