EphrinB (phospho Tyr298) Antibody

• Background:

  EphrinB proteins are thought to play key roles in cellular functions as diverse as neuronal migration and blood vessel development (Flanagan and Vancerhaeghen, 1998; Dufour et al., 2003; Oike et al., 2002) . EphrinB molecules expressed at the membrane surface bind to the EphB family receptors on target cells during cellto cell contact. This interaction leads to cell signaling in the target cell but also generates a reverse signal in the cell expressing EphrinB on its surface. This reverse signaling event is thought to be critical for vessel maturation and neuronal development. Importantly, tyrosine phosphorylation of EphrinB is thought to be a critical component of this reverse signaling event (Palmer et al., 2002) . Recent work suggests that phosphorylation of a specific EphrinB residue (Tyr298) plays a key role in EphrinB signaling (Kalo, et al., 2001) .

• NCBI Gene ID:

  396513

• Swiss Prot:

  P28693

• Accession Number:

  P28693

• Host:

  Rabbit

• Reactivity:

  Human, Mouse, Rat, Dog, Zebrafish, Bovine, Chicken, Frog

• Immunogen:

  Phosphopeptide corresponding to amino acid residues surrounding the phosphoTyr298 of Xenopus EphrinB. Note: Xenopus Tyr298 is the homolog of human, mouse and rat Tyr317 and also chicken Tyr305.

• Clonality:

  Polyclonal

• Conjugation:

  Unconjugated

• Type:

  Primary Antibodies

• Field of Research:

  Phospho-Specific

• Purification:

  Affinity Purified

• Concentration:

  Batch dependent

• Modification:

  Phospho-Specific

• Shipping Conditions:

  Blue Ice

• Storage Conditions:

  EphrinB antibody can be stored at -20˚ C and is stable at -20˚ C for at least 1 year.

• Calculated Molecular Weight:

  46

• Applications Notes:

  The antibody has been directly tested for reactivity in Western blots with rat tissue. It is anticipated that the antibody will react with bovine, canine, chicken, human, mouse, non-human primate, Xenopus and zebra fish based on the fact that these species have 100% homology with the amino acid sequence used as antigen.

• Symbol:

  EPHB2

• NCBI Official Name:

  EPH receptor B2

• NCBI Organism:

  Gallus gallus

• Background Reference 01:

  Bong, Y.S., Park, Y.H., Lee, H.S., Mood, K., Ishimura, A. and Daar, I.O. Tyr-298 in ephrinB1 is critical for an interaction with the Grb4 adaptor protein, Biochem. J. 377:499-507 (2004) .

• Background Reference 02:

  Dufour, A., Seibt, J., Passante, L., Depaepe, V., Ciossek, T., Frisen, J., Kullander, K., Flanagan, J.G., Polleux, F. and Vanderhaeghen, P. Area specificity and topography of thalamocortical projections are controlled by ephrin/Eph genes, Neuron 39:453-465 (2003) .

• Background Reference 03:

  Flanagan, J.G. and Vanderhaeghen, P. The ephrins and Eph receptors in neural development, Annu. Rev. Neurosci. 21:309-345 (1998) .

• Tested Applications:

  WB

• Protein ID:

  12230900

• Physical Properties:

  Liquid