Rabphilin (phospho Ser234) Antibody

• Background:

  Rabphilin-3A is a peripheral membrane protein that binds Ca2+ and phospholipids and is attached to synaptic vesicle membranes (Geppert et al., 1994; Li et al., 1994) . The expression of rabphilin 3A is reduced in an animal model of Huntington’s disease (Smith et al., 2005) . Rabphilin can be phosphorylated at Ser234 by both CAM kinase II and PKA and this phosphorylation has been suggested to regulate neuronal activity during development in a synapse-specific manner (Fykse et al., 1995; Foletti and Scheller, 2001) .

• NCBI Gene ID:

  171039

• Swiss Prot:

  P47709

• Accession Number:

  P47709

• Host:

  Rabbit

• Reactivity:

  Mouse, Rat

• Immunogen:

  Phosphopeptide corresponding to amino acid residues surrounding the phospho-Ser234 of rabphilin 3A.

• Clonality:

  Polyclonal

• Conjugation:

  Unconjugated

• Type:

  Primary Antibodies

• Field of Research:

  Phospho-Specific

• Purification:

  Affinity Purified

• Concentration:

  Batch dependent

• Modification:

  Phospho-Specific

• Shipping Conditions:

  Blue Ice

• Storage Conditions:

  Rabphilin antibody can be stored at -20˚ C and is stable at -20˚ C for at least 1 year.

• Calculated Molecular Weight:

  82

• Applications Notes:

  The antibody has been directly tested for reactivity in Western blots with rat tissue. It is anticipated that the antibody will react with mouse based on the fact that this species has 100% homology with the amino acid sequence used as antigen.

• Symbol:

  Rph3a

• NCBI Official Name:

  Rabphilin 3A

• NCBI Organism:

  Rattus norvegicus

• Background Reference 01:

  Foletti DL, Scheller RH (2001) Developmental regulation and specific brain distribution of phosphorabphilin. J Neurosci 21:5461-5472.

• Background Reference 02:

  Fykse EM, Li C, Sudhof TC (1995) Phosphorylation of rabphilin-3A by Ca2+/calmodulin- and cAMP-dependent protein kinases in vitro. J Neurosci 15:2385-2395.

• Background Reference 03:

  Geppert M, Bolshakov VY, Siegelbaum SA, Takei K, De Camilli P, Hammer RE, Südhof TC (1994) The role of Rab3A in neurotransmitter release. Nature (Lond) 369:493-497.

• Tested Applications:

  WB, IHC

• Protein ID:

  1350830

• Physical Properties:

  Liquid