GAPDH Antibody (biotin)

• Background:

  Glyceraldehyde-3-phosphate dehydrogenase (GAPDH) catalyzes the reversible oxidative phosphorylation of glyceraldehyde-3-phosphate in the presence of inorganic phosphate and nicotinamide adenine dinucleotide (NAD), an important energy-yielding step in carbohydrate metabolism. It also is involved in a number of cellular processes such as membrane fusion, phosphotransferase activity, DNA replication and repair, and nuclear RNA export (1) . GAPDH also plays a role in different pathologies such as cancer progression, apoptosis, and neuronal diseases such as Alzheimer's and Huntington's disease (2) . GAPDH is constitutively expressed at high levels in almost all tissues and cell lines making it ideal for use as a loading control marker in immunoblots.

• NCBI Gene ID:

  2597

• Swiss Prot:

  P04406

• Accession Number:

  NP_002037

• Host:

  Rabbit

• Reactivity:

  Human, Mouse, Rat, Rabbit, Chicken

• Immunogen:

  Biotin-GAPDH antibody was raised against a synthetic peptide containing 16 amino acids near the carboxy terminus of GAPDH.

• Clonality:

  Polyclonal

• Conjugation:

  Biotin

• Type:

  Primary Antibodies

• Field of Research:

  Signal Transduction, Neuroscience, Homeostasis

• Purification:

  Biotin-GAPDH antibody is affinity chromatography purified via peptide column.

• Positive Control:

  Cat. No. 1201 - HeLa Cell Lysate

• Concentration:

  1 mg/mL

• Buffer:

  Biotin-GAPDH antibody is supplied in PBS containing 1% BSA and 0.02% sodium azide.

• Modification:

  None

• Shipping Conditions:

  Blue Ice

• Storage Conditions:

  Biotin-GAPDH antibody can be stored at 4˚ C for three months and -20˚ C, stable for up to one year.

• Fragment:

  IgG

• Specificity:

  Biotin-GAPDH antibody is human, mouse, rat, rabbit, and chicken reactive.

• Symbol:

  GAPDH

• NCBI Official Name:

  Glyceraldehyde-3-phosphate dehydrogenase

• NCBI Organism:

  Homo sapiens

• Background Reference 01:

  Sirover MA. New nuclear functions of the glycolytic protein, glyceraldehyde-3-phosphate dehydrogenase, in mammalian cells. J. Cell. Biochem. 2005; 95:45-52.

• Background Reference 02:

  Glyceraldehyde-3-phosphate dehydrogenase, apoptosis, and neurodegenerative diseases. Annu. Rev. Pharmacol. Toxicol. 2005; 45:269-90.

• Other Product Names:

  Biotin-GAPDH, Glyceraldehyde-3-phosphate dehydrogenase, G3PDH, GAPD

• Tested Applications:

  ELISA, WB

• Protein ID:

  7669492

• Physical Properties:

  Liquid