Adiponectin Antibody

• Background:

  ACRP30 was identified as a novel adipocyte-specific synthesized and secreted protein with structural resemblance to complement factor C1q. Like adipsin, ACRP30 secretion is induced ~10-fold during adipocyte differentiation. Plasma levels are reduced in obese humans, and low levels are associated with insulin-resistance. Treatment of db/db mice with TZD increased ACRP30 levels.

• NCBI Gene ID:

  246253

• Swiss Prot:

  Q8K3R4

• Accession Number:

  81901098

• Host:

  Rabbit

• Reactivity:

  Rat

• Immunogen:

  Recombinant rat adiponectin.

• Clonality:

  Polyclonal

• Conjugation:

  Unconjugated

• Type:

  Primary Antibodies

• Field of Research:

  Obesity

• Concentration:

  1 mg/ml

• Buffer:

  Liquid. 0.2um-filtered solution in PBS, pH 7.4. Contains no preservatives.

• Modification:

  None

• Shipping Conditions:

  Blue Ice

• Storage Conditions:

  Stable for at least 6 months after receipt when stored at -20˚ C.

• Specificity:

  Recognizes rat adiponectin. Weakly cross-reacts with mouse adiponectin. Detects a band of ~35kDa by Western blot.

• Applications Notes:

  ELISA: (direct and indirect: 1:5,000-1:10,000) . Immunohistochemistry: (frozen sections (1:1,000) ) . Western Blot: (1:5,000-1:10,000 using ECL. Suggested blocking and dilution buffer is TBST containing 0.05% Tween 20 and 5% skim milk. Suggested incubation time is 1 hour at room temperature) . Optimal conditions must be determined individually for each application.

• Symbol:

  Adipoq

• NCBI Official Name:

  Adiponectin, C1Q and collagen domain containing

• NCBI Organism:

  Rattus norvegicus

• Other Product Names:

  ACRP30; AdipoQ; apM1; GBP28; Adipocyte Complement Related Protein of 30kDa

• Tested Applications:

  ELISA, IHC, WB

• Protein ID:

  Q8K3R4

• Physical Properties:

  Liquid