Adiponectin Antibody

• Background :

  ACRP30 was identified as a novel adipocyte-specific synthesized and secreted protein with structural resemblance to complement factor C1q. Like adipsin, ACRP30 secretion is induced ~10-fold during adipocyte differentiation. Plasma levels are reduced in obese humans, and low levels are associated with insulin-resistance. Treatment of db/db mice with TZD increased ACRP30 levels.

• NCBI Gene ID :

  246253

• Swiss Prot :

  Q8K3R4

• Accession Number :

  81901098

• Host :

  Rabbit

• Reactivity :

  Rat

• Immunogen :

  Recombinant rat adiponectin.

• Clonality :

  Polyclonal

• Conjugation :

  Unconjugated

• Type :

  Primary Antibodies

• Field of Research :

  Obesity

• Concentration :

  1 mg/ml

• Buffer :

  Liquid. 0.2um-filtered solution in PBS, pH 7.4. Contains no preservatives.

• Modification :

  None

• Shipping Conditions :

  Blue Ice

• Storage Conditions :

  Stable for at least 6 months after receipt when stored at -20˚ C.

• Specificity :

  Recognizes rat adiponectin. Weakly cross-reacts with mouse adiponectin. Detects a band of ~35kDa by Western blot.

• Applications Notes :

  ELISA: (direct and indirect: 1:5,000-1:10,000) . Immunohistochemistry: (frozen sections (1:1,000) ) . Western Blot: (1:5,000-1:10,000 using ECL. Suggested blocking and dilution buffer is TBST containing 0.05% Tween 20 and 5% skim milk. Suggested incubation time is 1 hour at room temperature) . Optimal conditions must be determined individually for each application.

• Symbol :

  Adipoq

• NCBI Official Name :

  Adiponectin, C1Q and collagen domain containing

• NCBI Organism :

  Rattus norvegicus

• Other Product Names :

  ACRP30; AdipoQ; apM1; GBP28; Adipocyte Complement Related Protein of 30kDa

• Tested Applications :

  ELISA, IHC, WB

• Protein ID :

  Q8K3R4

• Physical Properties :

  Liquid