Adiponectin Antibody

• Background:

  ACRP30 was identified as a novel adipocyte-specific synthesized and secreted protein with structural resemblance to complement factor C1q. Like adipsin, ACRP30 secretion is induced ~10-fold during adipocyte differentiation. Plasma levels are reduced in obese humans, and low levels are associated with insulin-resistance. Treatment of db/db mice with TZD increased ACRP30 levels.

• NCBI Gene ID:

  9370

• Swiss Prot:

  Q15848

• Accession Number:

  295317372

• Host:

  Rabbit

• Reactivity:

  Human

• Immunogen:

  Recombinant human adiponectin.

• Clonality:

  Polyclonal

• Conjugation:

  Unconjugated

• Type:

  Primary Antibodies

• Field of Research:

  Obesity

• Concentration:

  1 mg/ml

• Buffer:

  Liquid. 0.2um-filtered solution in PBS, pH 7.4. Contains no preservatives.

• Modification:

  None

• Shipping Conditions:

  Blue Ice

• Storage Conditions:

  Stable for at least 6 months after receipt when stored at -20˚ C.

• Specificity:

  Recognizes human adiponectin. Detects a band of ~30kDa by Western blot.

• Applications Notes:

  ELISA: (direct and indirect; 1:5,000-1:10,000) . Western Blot: (1:5,000-1:10,000 using ECL; suggested blocking and dilution buffer is TBST with 0.05% Tween 20 with 5% skim milk; incubate 1 hour at room temperature) . Optimal conditions must be determined individually for each application.

• Symbol:

  ADIPOQ

• NCBI Official Name:

  Adiponectin, C1Q and collagen domain containing

• NCBI Organism:

  Homo sapiens

• Other Product Names:

  ACRP30; AdipoQ; apM1; GBP28; Adipocyte Complement Related Protein of 30kDa

• Tested Applications:

  ELISA, WB

• Protein ID:

  NP_001171271.1

• Physical Properties:

  Liquid