E2F2 Antibody

• Background :

  E2F2 is a member of the E2F family of transcription factors. The E2F family plays a crucial role in the control of cell cycle and action of tumor suppressor proteins and is also a target of the transforming proteins of small DNA tumor viruses. The E2F proteins contain several evolutionally conserved domains found in most members of the family. These domains include a DNA binding domain, a dimerization domain which determines interaction with the differentiation regulated transcription factor proteins (DP), a transactivation domain enriched in acidic amino acids, and a tumor suppressor protein association domain which is embedded within the transactivation domain. This protein and another 2 members, E2F1 and E2F3, have an additional cyclin binding domain. This protein binds specifically to retinoblastoma protein pRB in a cell-cycle dependent manner, and it exhibits overall 46% amino acid identity to E2F1. [provided by RefSeq].

• NCBI Gene ID :

  1870

• Swiss Prot :

  Q14209

• Accession Number :

  Q14209

• Host :

  Rabbit

• Reactivity :

  Human, Mouse

• Immunogen :

  This E2F2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 258-286 amino acids from the Central region of human E2F2.

• Clonality :

  Polyclonal

• Conjugation :

  Unconjugated

• Type :

  Primary Antibodies

• Field of Research :

  Other

• Purification :

  This antibody is purified through a protein A column, followed by peptide affinity purification.

• Concentration :

  Batch dependent

• Buffer :

  Supplied in PBS with 0.09% (W/V) sodium azide.

• Modification :

  None

• Shipping Conditions :

  Blue Ice

• Storage Conditions :

  Store at 4˚ C for three months and -20˚ C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

• Calculated Molecular Weight :

  48 kDa

• Fragment :

  Rabbit Ig

• Applications Notes :

  For WB starting dilution is: 1:1000

• Symbol :

  E2F2

• NCBI Official Name :

  Transcription factor E2F2

• NCBI Organism :

  Homo sapiens

• Background Reference 01 :

  Revenko, A.S., et al. Mol. Cell. Biol. 30 (22) :5260-5272 (2010)

• Background Reference 02 :

  Hayami, S., et al. Mol. Cancer 9, 59 (2010) :

• Background Reference 03 :

  Chen, J., et al. Cancer Causes Control 20 (9) :1769-1777 (2009)

• Background Reference 04 :

  Cunningham, J.M., et al. Br. J. Cancer 101 (8) :1461-1468 (2009)

• Other Product Names :

  Transcription factor E2F2, E2F-2, E2F2

• Tested Applications :

  WB

• Protein ID :

  2494228

• Physical Properties :

  Liquid