Cytokeratin 7 Antibody [KRT7/903]

• Background:

  Cytokeratin 7 is a basic cytokeratin, which is found in most glandular and transitional epithelia but not in the stratified squamous epithelia. Keratin 7 is expressed in the epithelial cells of ovary, lung, and breast but not of colon, prostate, or gastrointestinal tract. Antibody to cytokeratin is useful in distinguishing ovarian carcinomas (keratin 7+) from colon carcinomas (keratin 7-) .

• NCBI Gene ID:

  3855

• Swiss Prot:

  P08729

• Host:

  Mouse

• Reactivity:

  Human

• Immunogen:

  Recombinant full-length human KRT7 protein was used as the immunogen for the Cytokeratin 7 antibody.

• Clonality:

  Monoclonal

• Clone:

  KRT7/903

• Conjugation:

  Unconjugated

• Type:

  Primary Antibodies

• Field of Research:

  Signal Transduction

• Purification:

  Protein G affinity chromatography

• Concentration:

  0.2 mg/mL

• Buffer:

  PBS with 0.1 mg/ml BSA and 0.05% sodium azide

• Modification:

  None

• Shipping Conditions:

  Blue Ice

• Storage Conditions:

  Aliquot and Store at 2-8˚ C. Avoid freez-thaw cycles.

• Fragment:

  IgG1

• Specificity:

  Does not react with Mouse, Rat, Ferret, rabbit

• Applications Notes:

  1. Staining of formalin-fixed tissues requires boiling tissue sections in 10mM Tris buffer with 1mM EDTA, pH 9.0, for 10-20 min followed by cooling at RT for 20 min 2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.

• Symbol:

  KRT7

• NCBI Official Name:

  Keratin 7

• NCBI Organism:

  Homo sapiens

• Other Product Names:

  Keratin, type II cytoskeletal 7, Cytokeratin-7, CK-7, Keratin-7, K7, Sarcolectin, Type-II keratin Kb7, KRT7, SCL

• Tested Applications:

  WB, Flow, IF, IHC-P

• Physical Properties:

  Liquid