Androgen Receptor Antibody [SPM335]

• Background:

  Recognizes a protein of 110kDa, which is identified as androgen receptor (AR) . It reacts with full length, and the newly described A form of the receptor. It does not cross react with estrogen, progesterone, or glucocorticoid receptors. The expression of AR is reportedly inversely correlated with histologic grade i.e. well differentiated prostate tumors show higher expression than the poorly differentiated tumors. In prostate cancer, AR has been proposed, as a marker of hormone-responsiveness and thus it may be useful in identifying patients likely to benefit from anti-androgen therapy. Anti-androgen receptor has been useful clinically in differentiating morpheaform basal cell carcinoma (mBCC) from desmoplastic trichoepithelioma (DTE) in the skin. This mAb is superb for staining of formalin/paraffin tissues.

• NCBI Gene ID:

  367

• Swiss Prot:

  P10275

• Host:

  Mouse

• Reactivity:

  Human

• Immunogen:

  Amino acids 302-318 (STEDTAEYSPFKGGYTK) from human AR were used as the immunogen for the anti-Androgen Receptor antibody.

• Clonality:

  Monoclonal

• Clone:

  SPM335

• Conjugation:

  Unconjugated

• Type:

  Primary Antibodies

• Field of Research:

  Cancer, Cell Cycle, Signal Transduction

• Purification:

  Protein G affinity chromatography

• Concentration:

  0.2 mg/mL

• Buffer:

  PBS with 0.1 mg/ml BSA and 0.05% sodium azide

• Modification:

  None

• Shipping Conditions:

  Blue Ice

• Storage Conditions:

  Aliquot and Store at 2-8˚ C. Avoid freez-thaw cycles.

• Fragment:

  IgG1, kappa

• Specificity:

  Does not react with Mouse

• Applications Notes:

  1. Staining of formalin-fixed tissues requires boiling tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 10-20 min followed by cooling at RT for 20 min 2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.

• Symbol:

  AR

• NCBI Official Name:

  Androgen receptor

• NCBI Organism:

  Homo sapiens

• Other Product Names:

  Androgen receptor, Dihydrotestosterone receptor, Nuclear receptor subfamily 3 group C member 4, AR, DHTR, NR3C4

• Tested Applications:

  Flow, IF, IHC-P

• Physical Properties:

  Liquid