Androgen Receptor (phospho Tyr363) Antibody

• Background:

  The androgen receptor gene is more than 90 kb long and codes for a protein that has 3 major functional domains: the N-terminal domain, DNA-binding domain, and androgen-binding domain. The protein functions as a steroid-hormone activated transcription factor. Upon binding the hormone ligand, the receptor dissociates from accessory proteins, translocates into the nucleus, dimerizes, and then stimulates transcription of androgen responsive genes. This gene contains 2 polymorphic trinucleotide repeat segments that encode polyglutamine and polyglycine tracts in the N-terminal transactivation domain of its protein. Expansion of the polyglutamine tract causes spinal bulbar muscular atrophy (Kennedy disease) . Mutations in this gene are also associated with complete androgen insensitivity (CAIS) . Two alternatively spliced variants encoding distinct isoforms have been described.

• NCBI Gene ID:

  367

• Swiss Prot:

  P10275

• Accession Number:

  NP_000035.2

• Host:

  Rabbit

• Reactivity:

  Human, Mouse

• Immunogen:

  Androgen Receptor (phospho Tyr363) antibody was raised against a peptide sequence around phosphorylation site of tyrosine 363 (D-Y-Y (p) -N-F) derived from Human Androgen Receptor.

• Clonality:

  Polyclonal

• Conjugation:

  Unconjugated

• Type:

  Primary Antibodies

• Field of Research:

  Phospho-Specific

• Purification:

  Antibodies were purified by affinity-chromatography using epitope-specific peptide.

• Concentration:

  1 mg/mL

• Buffer:

  Antibody supplied in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

• Modification:

  Phospho-Specific

• Shipping Conditions:

  Blue Ice

• Storage Conditions:

  Store antibody at -20˚ C for up to one year.

• Calculated Molecular Weight:

  85 kDa

• Fragment:

  IgG

• Specificity:

  The antibody detects endogenous levels of Androgen Receptor only when phosphorylated at tyrosine 363.

• Applications Notes:

  Western Blot: 1:500~1:1000

• Symbol:

  AR

• NCBI Official Name:

  Androgen receptor

• NCBI Organism:

  Homo sapiens

• Background Reference 01:

  Lubahn D.B., Mol. Endocrinol. 2:1265-1275 (1988) .

• Background Reference 02:

  Chang C., Proc. Natl. Acad. Sci. U.S.A. 85:7211-7215 (1988) .

• Background Reference 03:

  Tilley W.D., Proc. Natl. Acad. Sci. U.S.A. 86:327-331 (1989) .

• Other Product Names:

  ANDR, Androgen receptor, Dihydrotestosterone receptor, NR3C4

• Tested Applications:

  WB

• Protein ID:

  21322252

• Physical Properties:

  Liquid