CD32 Antibody [7.3]

• Background:

  This mAb reacts with a CD32 (FcgRII) epitope (cluster-4) . It displays a stronger reaction with Daudi than with U937 cells. The epitope is located in domain 2 of FcgRIIa. Its Fab'2 fragments block immune complex binding. CD32 (FcgRII) is a type 1 transmembrane glycoprotein that mediates several functions including phagocytosis, cytotoxicity, and immunomodulation as well as platelet aggregation. Three genes (A, B, and C) encode CD32 and at least 6 isoforms are generated via alternative mRNA splicing, i.e., IIa1, IIa2, IIb1, IIb2, IIb3 and IIc. Monocytes/macrophages, placental trophoblasts and endothelial cells express all isoforms. In addition, the IIb isoform is expressed by B cells, and the IIa isoform by platelets, granulocytes and, weakly, by B cells. NK cells and neutrophils express Isoform IIc. CD32 binds weakly to the Fc region of monomeric IgG but more strongly to IgG aggregates and immune complexes.

• NCBI Gene ID:

  2212

• Swiss Prot:

  P12318

• Host:

  Mouse

• Reactivity:

  Human

• Immunogen:

  K562 and FcgRII+L cells were used as the immunogen for the CD32 antibody.

• Clonality:

  Monoclonal

• Clone:

  7.3

• Conjugation:

  Unconjugated

• Type:

  Primary Antibodies

• Field of Research:

  Immunology, Signal Transduction

• Purification:

  Protein G affinity chromatography

• Concentration:

  0.2 mg/mL

• Buffer:

  PBS with 0.1 mg/ml BSA and 0.05% sodium azide

• Modification:

  None

• Shipping Conditions:

  Blue Ice

• Storage Conditions:

  Aliquot and Store at 2-8˚ C. Avoid freez-thaw cycles.

• Fragment:

  IgG1, kappa

• Symbol:

  FCGR2A

• NCBI Official Name:

  Fc fragment of IgG, low affinity IIa, receptor (CD32)

• NCBI Organism:

  Homo sapiens

• Other Product Names:

  Low affinity immunoglobulin gamma Fc region receptor II-a, IgG Fc receptor II-a, CDw32, Fc-gamma RII-a, Fc-gamma-RIIa, FcRII-a, CD32, FCGR2A, CD32, FCG2, FCGR2A1, IGFR2

• Tested Applications:

  WB, Functional Assays, Flow, IF

• Physical Properties:

  Liquid