EMA Antibody [MUC1/520]

• Background:

  This antibody recognizes proteins in MW range of 265-400kDa, identified as different glycoforms of MUC1 (Mucin-1) or EMA (epithelial membrane antigen) . The alpha subunit has cell adhesive properties. It can act both as an adhesion and an anti-adhesion protein. MUC1 / Mucin-1 may provide a protective layer on epithelial cells against bacterial and enzyme attack. The beta subunit contains a C-terminal domain, which is involved in cell signaling through phosphorylations and protein-protein interactions. In immunohistochemical assays, the MUC1 / EMA antibody superbly stains routine formalin/paraffin carcinoma tissues. MUC1 antibody is useful as a pan-epithelial marker for detecting early metastatic loci of carcinoma in bone marrow or liver.

• NCBI Gene ID:

  4582

• Swiss Prot:

  P15941

• Host:

  Mouse

• Reactivity:

  Human

• Immunogen:

  Human milk fat globule membranes were used as the immunogen for this EMA antibody.

• Clonality:

  Monoclonal

• Clone:

  MUC1/845

• Conjugation:

  Unconjugated

• Type:

  Primary Antibodies

• Field of Research:

  Signal Transduction

• Purification:

  Protein G affinity chromatography

• Concentration:

  0.2 mg/mL

• Buffer:

  PBS with 0.1 mg/ml BSA and 0.05% sodium azide

• Modification:

  None

• Shipping Conditions:

  Blue Ice

• Storage Conditions:

  Aliquot and Store at 2-8˚ C. Avoid freez-thaw cycles.

• Fragment:

  IgG1, kappa

• Applications Notes:

  1. No pretreatment needed for FFPE staining. 2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.

• Symbol:

  MUC1

• NCBI Official Name:

  Mucin 1, cell surface associated

• NCBI Organism:

  Homo sapiens

• Other Product Names:

  MUC1 Antibody : EMA, MCD, PEM, PUM, KL-6, MAM6, MCKD, PEMT, CD227, H23AG, MCKD1, MUC-1, ADMCKD, ADMCKD1, CA 15-3, MUC-1/X, MUC1/ZD, MUC-1/SEC, Mucin-1, Breast carcinoma-associated antigen DF3

• Tested Applications:

  WB, Flow, IHC, IF

• Physical Properties:

  Liquid