Thymidylate Synthase Antibody [TMS715]

• Background:

  This antibody is specific for a protein of 36kDa, identified as Thymidylate Synthase (TS) . Thymidylate synthase converts deoxyuridine monophosphate (dUMP) to deoxythymidine monophosphate (dTMP), which is essential for DNA biosynthesis. Thymidylate synthase is also a critical target for the fluoropyrimidines, an important group of anti-neoplastic drugs that are widely used in the treatment of solid tumors. Both 5-FU and fluorodeoxyuridine are converted in tumor cells to FdUMP which inactivates thymidylate synthase by formation of a ternary covalent complex in the presence of the folate cofactor 5,10-methylenetetrahydrofolate. Expression of thymidylate synthase protein is associated with response to 5-fluorouracil (5-FU) in human colorectal, gastric, head and neck, and breast carcinomas.

• NCBI Gene ID:

  7298

• Swiss Prot:

  P04818

• Host:

  Mouse

• Reactivity:

  Human

• Immunogen:

  Recombinant human Thymidylate synthase was used as the immunogen for this antibody.

• Clonality:

  Monoclonal

• Clone:

  TMS715

• Conjugation:

  Unconjugated

• Type:

  Primary Antibodies

• Field of Research:

  Cancer

• Purification:

  Protein G purified monoclonal antibody

• Concentration:

  0.2 mg/mL

• Buffer:

  PBS with 0.1 mg/ml BSA and 0.05% sodium azide

• Modification:

  None

• Shipping Conditions:

  Blue Ice

• Storage Conditions:

  Aliquot and Store at 2-8˚ C. Avoid freez-thaw cycles.

• Fragment:

  IgG1, kappa

• Applications Notes:

  1. Staining of formalin-fixed tissues is enhanced by boiling tissue sections in 10mM Citrate Buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes. 2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.

• Symbol:

  TYMS

• NCBI Official Name:

  Thymidylate synthetase

• NCBI Organism:

  Homo sapiens

• Other Product Names:

  TYMS, HsT422, MGC88736, TMS, TS, TSase, HST422

• Tested Applications:

  Flow, IHC, IF

• Physical Properties:

  Liquid