Beta-Catenin Antibody

• Background:

  Beta-catenin associates with the cytoplasmic portion of E-cadherin, which is necessary for the function of E-cadherin as an adhesion molecule. In normal tissues, beta-catenin is localized to the membrane of epithelial cells, consistent with its role in the cell adhesion complex. In breast ductal neoplasia, it is usually localized in cellular membranes. However, in lobular neoplasia, a marked redistribution throughout the cytoplasm results in a diffuse cytoplasmic pattern. Staining with beta-catenin antibody and E-cadherin antibody helps in the accurate identification of ductal and lobular neoplasms, including a distinction between low-grade ductal carcinoma in situ (DCIS) and lobular carcinoma. Additionally, some rectal and gastric adenocarcinomas demonstrate diffuse cytoplasmic staining and a lack of membranous staining, mimicking the staining pattern observed with lobular breast carcinomas.

• NCBI Gene ID:

  1499

• Swiss Prot:

  P35222

• Host:

  Rabbit

• Reactivity:

  Human, Mouse

• Immunogen:

  A synthetic peptide from the middle of beta-Catenin / p120 protein was used as the immunogen.

• Clonality:

  Polyclonal

• Conjugation:

  Unconjugated

• Type:

  Primary Antibodies

• Field of Research:

  Cancer, Neuroscience, Signal Transduction

• Purification:

  Protein A affinity chromatography

• Concentration:

  0.2 mg/mL

• Buffer:

  PBS with 0.1 mg/ml BSA and 0.05% sodium azide

• Modification:

  None

• Shipping Conditions:

  Blue Ice

• Storage Conditions:

  Aliquot and Store at 2-8˚ C. Avoid freez-thaw cycles.

• Fragment:

  IgG

• Applications Notes:

  1. Staining of formalin-fixed tissues requires boiling tissue sections in 1mM EDTA, pH 9.0, for 10-20 min followed by cooling at RT for 20 minutes. 2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.

• Symbol:

  CTNNB1

• NCBI Official Name:

  Catenin (cadherin-associated protein), beta 1, 88kDa

• NCBI Organism:

  Homo sapiens

• Other Product Names:

  Catenin beta-1, Beta-catenin, CTNNB1, CTNNB

• Tested Applications:

  WB, Flow, IF, IHC

• Physical Properties:

  Liquid