Prostate Specific Antigen Antibody [A67-B/E3]

• Background:

  This antibody recognizes a single protein of 33-34kDa, identified as the Prostate specific antigen. This PSA antibody is highly specific and stains prostatic secretory and ductal epithelium in both normal and neoplastic tissues. Prostate specific antigen is a chymotrypsin-like serine protease (kallikrein family) exclusively produced by the prostate epithelium, and abundant in seminal fluid. PSA can be detected in the sera of patients with prostatic carcinoma. It is predominantly complexed to a liver-derived serine protease inhibitor, alpha-1-antichymotrypsin (ACT) . A higher proportion of serum PSA is complexed to ACT in prostate cancer than in benign hyperplasia.

• NCBI Gene ID:

  354

• Swiss Prot:

  P07288

• Host:

  Mouse

• Reactivity:

  Human

• Immunogen:

  Prostate Specific Antigen from human sperm plasma was used as the antigen for this PSA antibody.

• Clonality:

  Monoclonal

• Clone:

  A67-B/E3

• Conjugation:

  Unconjugated

• Type:

  Primary Antibodies

• Field of Research:

  Cancer, Cell Cycle, Neuroscience, Signal Transduction

• Purification:

  Protein G affinity chromatography

• Concentration:

  0.2 mg/mL

• Buffer:

  PBS with 0.1 mg/ml BSA and 0.05% sodium azide

• Modification:

  None

• Shipping Conditions:

  Blue Ice

• Storage Conditions:

  Aliquot and Store at 2-8˚ C. Avoid freez-thaw cycles.

• Fragment:

  IgG1, kappa

• Applications Notes:

  1. Staining of formalin-fixed tissues requires boiling tissue sections in 1mM EDTA Buffer, pH 7.5-8.5, for 10-20 min followed by cooling at RT for 20 minutes. 2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.

• Symbol:

  KLK3

• NCBI Official Name:

  Kallikrein-related peptidase 3

• NCBI Organism:

  Homo sapiens

• Other Product Names:

  Prostate-specific antigen, PSA, Gamma-seminoprotein, Seminin, Kallikrein-3, P-30 antigen, Semenogelase, KLK3, APS

• Tested Applications:

  WB, Flow, IHC, IF

• Physical Properties:

  Liquid