MGAT2 Antibody

• Background:

  MGAT2 is a Golgi enzyme catalyzing an essential step in the conversion of oligomannose to complex N-glycans. The enzyme has the typical glycosyltransferase domains: a short N-terminal cytoplasmic domain, a hydrophobic non-cleavable signal-anchor domain, and a C-terminal catalytic domain. Mutations in its gene may lead to carbohydrate-deficient glycoprotein syndrome, type II.The product of this gene is a Golgi enzyme catalyzing an essential step in the conversion of oligomannose to complex N-glycans. The enzyme has the typical glycosyltransferase domains: a short N-terminal cytoplasmic domain, a hydrophobic non-cleavable signal-anchor domain, and a C-terminal catalytic domain. Mutations in this gene may lead to carbohydrate-deficient glycoprotein syndrome, type II. Two transcript variants encoding the same protein have been identified for this gene.

• NCBI Gene ID:

  4247

• Swiss Prot:

  Q10469

• Accession Number:

  NP_002399

• Host:

  Rabbit

• Reactivity:

  Human, Mouse, Rat, Dog

• Immunogen:

  Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of human MGAT2.

• Clonality:

  Polyclonal

• Conjugation:

  Unconjugated

• Type:

  Primary Antibodies

• Field of Research:

  Membrane, Signal Transduction

• Purification:

  Antibody is purified by protein A chromatography method.

• Positive Control:

  Cat. No. 1205 - Jurkat Cell Lysate

• Concentration:

  Batch dependent

• Buffer:

  Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose.

• Modification:

  None

• Shipping Conditions:

  Blue Ice

• Storage Conditions:

  For short periods of storage (days) store at 4˚ C. For longer periods of storage, store MGAT2 antibody at -20˚ C. As with any antibody avoid repeat freeze-thaw cycles.

• Calculated Molecular Weight:

  51 kDa

• Applications Notes:

  MGAT2 antibody can be used for detection of MGAT2 by ELISA at 1:62500. MGAT2 antibody can be used for detection of MGAT2 by western blot at 1.25 μ g/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.

• Symbol:

  MGAT2

• NCBI Official Name:

  Mannosyl (alpha-1,6-) -glycoprotein beta-1,2-N-acetylglucosaminyltransferase

• NCBI Organism:

  Homo sapiens

• Background Reference 01:

  Otsuki, T., (2005) DNA Res. 12 (2), 117-126.

• Other Product Names:

  MGAT2, CDGS2, GLCNACTII, GNT-II, GNT2, CDG2A

• Tested Applications:

  ELISA, WB, IHC

• Protein ID:

  4505163

• Physical Properties:

  Liquid