E2F5 Antibody

• Background:

  E2F5 is a member of the E2F family of transcription factors. The E2F family plays a crucial role in the control of cell cycle and action of tumor suppressor proteins and is also a target of the transforming proteins of small DNA tumor viruses. The E2F proteins contain several evolutionally conserved domains found in most members of the family. These domains include a DNA binding domain, a dimerization domain which determines interaction with the differentiation regulated transcription factor proteins (DP), a transactivation domain enriched in acidic amino acids, and a tumor suppressor protein association domain which is embedded within the transactivation domain. This protein is differentially phosphorylated and is expressed in a wide variety of human tissues. It is more homologous to E2F4, a family member, than to other members. Both this protein and E2F4 interact with tumor suppressor proteins p130 and p107, but not with pRB.

• NCBI Gene ID:

  1875

• Swiss Prot:

  Q15329

• Accession Number:

  NP_001942

• Host:

  Rabbit

• Reactivity:

  Human

• Immunogen:

  Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of human E2F5.

• Clonality:

  Polyclonal

• Conjugation:

  Unconjugated

• Type:

  Primary Antibodies

• Field of Research:

  Transcription, Cell Cycle, Cancer

• Purification:

  Antibody is purified by peptide affinity chromatography method.

• Positive Control:

  Cat. No. 1205 - Jurkat Cell Lysate

• Concentration:

  Batch dependent

• Buffer:

  Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose.

• Modification:

  None

• Shipping Conditions:

  Blue Ice

• Storage Conditions:

  For short periods of storage (days) store at 4˚ C. For longer periods of storage, store E2F5 antibody at -20˚ C. As with any antibody avoid repeat freeze-thaw cycles.

• Calculated Molecular Weight:

  38 kDa

• Applications Notes:

  E2F5 antibody can be used for detection of E2F5 by ELISA at 1:1562500. E2F5 antibody can be used for detection of E2F5 by western blot at 0.4 μ g/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.

• Symbol:

  E2F5

• NCBI Official Name:

  E2F transcription factor 5, p130-binding

• NCBI Organism:

  Homo sapiens

• Background Reference 01:

  Ohtani, N., et al., (2003) J. Cell Biol. 162 (2), 173-183.

• Other Product Names:

  E2F5, E2F-5

• Tested Applications:

  ELISA, WB

• Protein ID:

  134142811

• Physical Properties:

  Liquid