E2F1 Antibody

• Background:

  The protein encoded by E2F1 is a member of the E2F family of transcription factors. The E2F family plays a crucial role in the control of cell cycle and action of tumor suppressor proteins and is also a target of the transforming proteins of small DNA tumor viruses. This protein binds preferentially to retinoblastoma protein pRB in a cell-cycle dependent manner. It can mediate both cell proliferation and p53-dependent/independent apoptosis.

• NCBI Gene ID:

  1869

• Swiss Prot:

  Q01094

• Accession Number:

  NP_005216

• Host:

  Rabbit

• Reactivity:

  Human

• Immunogen:

  Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of human E2F1.

• Clonality:

  Polyclonal

• Conjugation:

  Unconjugated

• Type:

  Primary Antibodies

• Field of Research:

  Transcription, Cell Cycle, Cancer, Apoptosis

• Purification:

  Antibody is purified by peptide affinity chromatography method.

• Positive Control:

  Cat. No. 1224 - Daudi Cell Lysate

• Concentration:

  Batch dependent

• Buffer:

  Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose.

• Modification:

  None

• Shipping Conditions:

  Blue Ice

• Storage Conditions:

  For short periods of storage (days) store at 4˚ C. For longer periods of storage, store E2F1 antibody at -20˚ C. As with any antibody avoid repeat freeze-thaw cycles.

• Calculated Molecular Weight:

  47 kDa

• Applications Notes:

  E2F1 antibody can be used for detection of E2F1 by ELISA at 1:312500. E2F1 antibody can be used for detection of E2F1 by western blot at 0.5 μ g/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.

• Symbol:

  E2F1

• NCBI Official Name:

  E2F transcription factor 1

• NCBI Organism:

  Homo sapiens

• Background Reference 01:

  Unoki, M., et al., (2004) Oncogene 23 (46), 7601-7610.

• Other Product Names:

  E2F1, RBP3, E2F-1, RBAP1, RBBP3

• Tested Applications:

  ELISA, WB

• Protein ID:

  12669911

• Physical Properties:

  Liquid