PRDX3 Antibody

• Background :

  PRDX3 is a protein with antioxidant function and is localized in the mitochondrion. Expression of this gene product in E. coli deficient in the C22-subunit gene rescued resistance of the bacteria to alkylhydroperoxide. The human and mouse genes are highly conserved, and they map to the regions syntenic between mouse and human chromosomes. Sequence comparisons with recently cloned mammalian homologues suggest that these genes consist of a family that is responsible for regulation of cellular proliferation, differentiation, and antioxidant functions. This gene encodes a protein with antioxidant function and is localized in the mitochondrion. This gene shows significant nucleotide sequence similarity to the gene coding for the C22 subunit of Salmonella typhimurium alkylhydroperoxide reductase. Expression of this gene product in E. coli deficient in the C22-subunit gene rescued resistance of the bacteria to alkylhydroperoxide. The human and mouse genes are highly conserved, and they map to the regions syntenic between mouse and human chromosomes. Sequence comparisons with recently cloned mammalian homologues suggest that these genes consist of a family that is responsible for regulation of cellular proliferation, differentiation, and antioxidant functions. Two transcript variants encoding two different isoforms have been found for this gene.

• NCBI Gene ID :

  10935

• Swiss Prot :

  P30048

• Accession Number :

  NP_054817

• Host :

  Rabbit

• Reactivity :

  Human

• Immunogen :

  Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of human PRDX3.

• Clonality :

  Polyclonal

• Conjugation :

  Unconjugated

• Type :

  Primary Antibodies

• Field of Research :

  Cancer

• Purification :

  Antibody is purified by peptide affinity chromatography method.

• Positive Control :

  Cat. No. 1205 - Jurkat Cell Lysate

• Concentration :

  Batch dependent

• Buffer :

  Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose.

• Modification :

  None

• Shipping Conditions :

  Blue Ice

• Storage Conditions :

  For short periods of storage (days) store at 4˚ C. For longer periods of storage, store PRDX3 antibody at -20˚ C. As with any antibody avoid repeat freeze-thaw cycles.

• Calculated Molecular Weight :

  26 kDa

• Applications Notes :

  PRDX3 antibody can be used for detection of PRDX3 by ELISA at 1:312500. PRDX3 antibody can be used for detection of PRDX3 by western blot at 0.5 μ g/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.

• Symbol :

  PRDX3

• NCBI Official Name :

  Peroxiredoxin 3

• NCBI Organism :

  Homo sapiens

• Background Reference 01 :

  Chiribau, C.B., (2008) J. Biol. Chem. 283 (13), 8211-8217.

• Other Product Names :

  PRDX3, AOP-1, AOP1, MER5, MGC104387, MGC24293, PRO1748, SP-22, HBC189, prx-III

• Tested Applications :

  ELISA, WB

• Protein ID :

  32483377

• Physical Properties :

  Liquid