CHST14 Antibody

• Background:

  CHST14 belongs to the sulfotransferase 2 family. CHST14 catalyzes the transfer of sulfate to position 4 of the N-acetylgalactosamine (GalNAc) residue of dermatan sulfate. It transfers sulfate to the C-4 hydroxyl of beta1,4-linked GalNAc that is substituted with an alpha-linked iduronic acid (IdoUA) at the C-3 hydroxyl. It transfers sulfate more efficiently to GalNAc residues in -IdoUA-GalNAc-IdoUA- than in -GlcUA-GalNAc-GlcUA-sequences. CHST14 has preference for partially desulfated dermatan sulfate. Addition of sulfate to GalNAc may occur immediately after epimerization of GlcUA to IdoUA.

• NCBI Gene ID:

  113189

• Swiss Prot:

  Q8NCH0

• Accession Number:

  AAH23653

• Host:

  Rabbit

• Reactivity:

  Human, Mouse, Rat

• Immunogen:

  Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of human CHST14.

• Clonality:

  Polyclonal

• Conjugation:

  Unconjugated

• Type:

  Primary Antibodies

• Field of Research:

  Other

• Purification:

  Antibody is purified by peptide affinity chromatography method.

• Positive Control:

  Cat. No. 1211 - HepG2 Cell Lysate

• Concentration:

  Batch dependent

• Buffer:

  Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose.

• Modification:

  None

• Shipping Conditions:

  Blue Ice

• Storage Conditions:

  For short periods of storage (days) store at 4˚ C. For longer periods of storage, store CHST14 antibody at -20˚ C. As with any antibody avoid repeat freeze-thaw cycles.

• Calculated Molecular Weight:

  35 kDa

• Applications Notes:

  CHST14 antibody can be used for detection of CHST14 by ELISA at 1:312500. CHST14 antibody can be used for detection of CHST14 by western blot at 1 μ g/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.

• Symbol:

  CHST14

• NCBI Official Name:

  Carbohydrate (N-acetylgalactosamine 4-0) sulfotransferase 14

• NCBI Organism:

  Homo sapiens

• Other Product Names:

  CHST14, D4ST-1, D4ST1, HD4ST, HNK1ST, ATCS, EDSMC1

• Tested Applications:

  ELISA, WB

• Protein ID:

  40289703

• Physical Properties:

  Liquid