YWHAZ Antibody

• Background:

  YWHAZ belongs to the 14-3-3 family of proteins which mediate signal transduction by binding to phosphoserine-containing proteins. This highly conserved protein family is found in both plants and mammals, and this protein is 99% identical to the mouse, rat and sheep orthologs. The protein interacts with IRS1 protein, suggesting a role in regulating insulin sensitivity.This gene product belongs to the 14-3-3 family of proteins which mediate signal transduction by binding to phosphoserine-containing proteins. This highly conserved protein family is found in both plants and mammals, and this protein is 99% identical to the mouse, rat and sheep orthologs. The encoded protein interacts with IRS1 protein, suggesting a role in regulating insulin sensitivity. Two transcript variants differing in the 5' UTR, but encoding the same protein, have been identified for this gene.

• NCBI Gene ID:

  7534

• Swiss Prot:

  P63104

• Accession Number:

  NP_003397

• Host:

  Rabbit

• Reactivity:

  Human, Mouse, Rat, Dog

• Immunogen:

  Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of human YWHAZ.

• Clonality:

  Polyclonal

• Conjugation:

  Unconjugated

• Type:

  Primary Antibodies

• Field of Research:

  Cancer

• Purification:

  Antibody is purified by protein A chromatography method.

• Positive Control:

  Cat. No. 1211 - HepG2 Cell Lysate

• Concentration:

  Batch dependent

• Buffer:

  Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose.

• Modification:

  None

• Shipping Conditions:

  Blue Ice

• Storage Conditions:

  For short periods of storage (days) store at 4˚ C. For longer periods of storage, store YWHAZ antibody at -20˚ C. As with any antibody avoid repeat freeze-thaw cycles.

• Calculated Molecular Weight:

  28 kDa, 28 kDa

• Applications Notes:

  YWHAZ antibody can be used for detection of YWHAZ by ELISA at 1:62500. YWHAZ antibody can be used for detection of YWHAZ by western blot at 5.0 μ g/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.

• Symbol:

  YWHAZ

• NCBI Official Name:

  Tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein, zeta

• NCBI Organism:

  Homo sapiens

• Background Reference 01:

  Coblitz, B., (2005) J. Biol. Chem. 280 (43), 36263-36272.

• Other Product Names:

  YWHAZ, KCIP-1, MGC111427, MGC126532, MGC138156, HEL4, YWHAD, HEL-S-3, 14-3-3-zeta

• Tested Applications:

  ELISA, WB

• Protein ID:

  4507953

• Physical Properties:

  Liquid