ZNF446 Antibody

• Background:

  ZNF446 contains a KRAB and three C (2) H (2) zinc fingers. ZNF446 is a transcription repressor when fused to GAL4 DNA-binding domain and co-transfected with VP-16. Overexpression of ZNF446 in COS-7 cells inhibits the transcriptional activities of SRE and AP-1, suggesting that the ZNF446 protein may act as a transcriptional repressor in mitogen-activated protein kinase (MAPK) signaling pathway to mediate cellular functions.

• NCBI Gene ID:

  55663

• Swiss Prot:

  Q9NWS9

• Accession Number:

  NP_060378

• Host:

  Rabbit

• Reactivity:

  Human

• Immunogen:

  Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of human ZNF446.

• Clonality:

  Polyclonal

• Conjugation:

  Unconjugated

• Type:

  Primary Antibodies

• Field of Research:

  Transcription

• Purification:

  Antibody is purified by protein A chromatography method.

• Positive Control:

  Cat. No. 1211 - HepG2 Cell Lysate

• Concentration:

  Batch dependent

• Buffer:

  Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose.

• Modification:

  None

• Shipping Conditions:

  Blue Ice

• Storage Conditions:

  For short periods of storage (days) store at 4˚ C. For longer periods of storage, store ZNF446 antibody at -20˚ C. As with any antibody avoid repeat freeze-thaw cycles.

• Calculated Molecular Weight:

  49 kDa

• Applications Notes:

  ZNF446 antibody can be used for detection of ZNF446 by ELISA at 1:1562500. ZNF446 antibody can be used for detection of ZNF446 by western blot at 1.25 μ g/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.

• Symbol:

  ZNF446

• NCBI Official Name:

  Zinc finger protein 446

• NCBI Organism:

  Homo sapiens

• Background Reference 01:

  Liu, F., et al., (2005) Biochem. Biophys. Res. Commun. 333 (1), 5-13.

• Other Product Names:

  ZNF446, ZSCAN30, ZSCAN52, ZKSCAN20

• Tested Applications:

  ELISA, WB

• Protein ID:

  8923582

• Physical Properties:

  Liquid