IKZF3 Antibody

• Background:

  IKZF3 is a member of the Ikaros family of zinc-finger proteins. It is a transcription factor that is important in the regulation of B lymphocyte proliferation and differentiation. Both Ikaros and Aiolos can participate in chromatin remodeling. Regulation of gene expression in B lymphocytes by Aiolos is complex as it appears to require the sequential formation of Ikaros homodimers, Ikaros/Aiolos heterodimers, and Aiolos homodimers.This gene encodes a member of the Ikaros family of zinc-finger proteins. Three members of this protein family (Ikaros, Aiolos and Helios) are hematopoetic-specific transcription factors involved in the regulation of lymphocyte development. This gene product is a transcription factor that is important in the regulation of B lymphocyte proliferation and differentiation. Both Ikaros and Aiolos can participate in chromatin remodeling. Regulation of gene expression in B lymphocytes by Aiolos is complex as it appears to require the sequential formation of Ikaros homodimers, Ikaros/Aiolos heterodimers, and Aiolos homodimers. At least six alternative transcripts encoding different isoforms have been described.

• NCBI Gene ID:

  22806

• Swiss Prot:

  Q9UKT9-2

• Accession Number:

  NP_899051

• Host:

  Rabbit

• Reactivity:

  Human

• Immunogen:

  Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of human IKZF3.

• Clonality:

  Polyclonal

• Conjugation:

  Unconjugated

• Type:

  Primary Antibodies

• Field of Research:

  Transcription, Cancer

• Positive Control:

  Cat. No. 1211 - HepG2 Cell Lysate

• Concentration:

  Batch dependent

• Buffer:

  Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose.

• Modification:

  None

• Shipping Conditions:

  Blue Ice

• Storage Conditions:

  For short periods of storage (days) store at 4˚ C. For longer periods of storage, store IKZF3 antibody at -20˚ C. As with any antibody avoid repeat freeze-thaw cycles.

• Calculated Molecular Weight:

  52 kDa, 58 kDa, 53 kDa, 54 kDa, 47 kDa, 49 kDa

• Applications Notes:

  IKZF3 antibody can be used for detection of IKZF3 by ELISA at 1:312500. IKZF3 antibody can be used for detection of IKZF3 by western blot at 0.25 μ g/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.

• Symbol:

  IKZF3

• NCBI Official Name:

  IKAROS family zinc finger 3 (Aiolos)

• NCBI Organism:

  Homo sapiens

• Background Reference 01:

  Koipally, J. (2002) J. Biol. Chem. 277 (31), 27697-27705.

• Other Product Names:

  IKZF3, AIO, AIOLOS, ZNFN1A3, RP11-94L15.2

• Tested Applications:

  ELISA, WB, IHC

• Protein ID:

  38045959

• Physical Properties:

  Liquid