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ZNF265 Antibody

CAT: 0223-28-738Size: 100 µLDry Ice: NoHazardous: No
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CAT#:0223-28-738Size:100 µL
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Background
ZNF265 is a protein that has been shown to bind to the spliceosomal components U1-70K and U2AF35 and to direct alternative splicing. Analysis of the structure reveals substantial similarity to known RNA-binding motifs in terms of the distribution of key surface residues responsible for making RNA contacts, despite a complete lack of structural homology. An RNA gel shift assay was used to demonstrate that a single crossed finger domain from ZNF265 is capable of binding to an RNA message. Taken together, these results define a new RNA-binding motif and should provide insight into the functions of the >100 uncharacterized proteins in the sequence data bases that contain this domain.
NCBI Gene ID
9406
Swiss Prot
Q5VV34
Accession Number
NP_976225
Host
Rabbit
Reactivity
Human, Mouse, Rat, Dog, Zebrafish
Immunogen
Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of human ZNF265.
Clonality
Polyclonal
Conjugation
Unconjugated
Type
Primary Antibodies
Field of Research
Transcription
Purification
Antibody is purified by peptide affinity chromatography method.
Positive Control
Cat. No. 1211 - HepG2 Cell Lysate
Concentration
Batch dependent
Buffer
Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose.
Modification
None
Shipping Conditions
Blue Ice
Storage Conditions
For short periods of storage (days) store at 4˚ C. For longer periods of storage, store ZNF265 antibody at -20˚ C. As with any antibody avoid repeat freeze-thaw cycles.
Calculated Molecular Weight
37 kDa, 36 kDa
Applications Notes
ZNF265 antibody can be used for detection of ZNF265 by ELISA at 1:312500. ZNF265 antibody can be used for detection of ZNF265 by western blot at 1.25 μ g/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.
Symbol
ZRANB2
NCBI Official Name
Zinc finger, RAN-binding domain containing 2
NCBI Organism
Homo sapiens
Background Reference 01
Plambeck, C.A., et al., (2003) J. Biol. Chem. 278 (25), 22805-22811.
Other Product Names
ZNF265, ZIS, ZIS1, ZIS2, ZNF265
Tested Applications
ELISA, WB
Protein ID
42741684
Physical Properties
Liquid

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