CHRNB2 Antibody

• Background:

  Mutations in nAChRs are found in a rare form of nocturnal frontal lobe epilepsy . Previously, some nAChR mutations have been described that are associated with additional neurological features such as psychiatric disorders or cognitive defects. A new CHRNB2 mutation located in transmembrane region 3 (M3), outside the known ADNFLE mutation cluster. The CHRNB2 mutation I312M, which occurred de novo in twins, markedly increases the receptor's sensitivity to acetylcholine. Phenotypically, the mutation is associated not only with typical ADNFLE, but also with distinct deficits in memory. The cognitive problems are most obvious in tasks requiring the organization and storage of verbal information.

• NCBI Gene ID:

  1141

• Swiss Prot:

  P17787

• Accession Number:

  NP_000739

• Host:

  Rabbit

• Reactivity:

  Human

• Immunogen:

  Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of human CHRNB2.

• Clonality:

  Polyclonal

• Conjugation:

  Unconjugated

• Type:

  Primary Antibodies

• Field of Research:

  Membrane, Signal Transduction

• Purification:

  Antibody is purified by peptide affinity chromatography method.

• Positive Control:

  Cat. No. 1211 - HepG2 Cell Lysate

• Concentration:

  Batch dependent

• Buffer:

  Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose.

• Modification:

  None

• Shipping Conditions:

  Blue Ice

• Storage Conditions:

  For short periods of storage (days) store at 4˚ C. For longer periods of storage, store CHRNB2 antibody at -20˚ C. As with any antibody avoid repeat freeze-thaw cycles.

• Calculated Molecular Weight:

  57 kDa

• Applications Notes:

  CHRNB2 antibody can be used for detection of CHRNB2 by ELISA at 1:312500. CHRNB2 antibody can be used for detection of CHRNB2 by western blot at 5.0 μ g/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.

• Symbol:

  CHRNB2

• NCBI Official Name:

  Cholinergic receptor, nicotinic, beta 2 (neuronal)

• NCBI Organism:

  Homo sapiens

• Background Reference 01:

  Feng, Y., et al., (2004) Am. J. Hum. Genet. 75 (1), 112-121.

• Other Product Names:

  CHRNB2, EFNL3, nAChRB2

• Tested Applications:

  ELISA, WB

• Protein ID:

  4502833

• Physical Properties:

  Liquid