GPC3 Antibody

• Background:

  Cell surface heparan sulfate proteoglycans are composed of a membrane-associated protein core substituted with a variable number of heparan sulfate chains. Members of the glypican-related integral membrane proteoglycan family (GRIPS) contain a core protein anchored to the cytoplasmic membrane via a glycosyl phosphatidylinositol linkage. These proteins may play a role in the control of cell division and growth regulation. Deletion mutations in this gene are associated with Simpson-Golabi-Behmel syndrome.

• NCBI Gene ID:

  2719

• Swiss Prot:

  P51654

• Accession Number:

  NP_004475

• Host:

  Rabbit

• Reactivity:

  Human, Mouse, Rat, Dog

• Immunogen:

  Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of human GPC3.

• Clonality:

  Polyclonal

• Conjugation:

  Unconjugated

• Type:

  Primary Antibodies

• Field of Research:

  Cancer, Signal Transduction

• Purification:

  Antibody is purified by protein A chromatography method.

• Positive Control:

  Cat. No. 1309 - Human Placenta Lysate

• Concentration:

  Batch dependent

• Buffer:

  Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose.

• Modification:

  None

• Shipping Conditions:

  Blue Ice

• Storage Conditions:

  For short periods of storage (days) store at 4˚ C. For longer periods of storage, store GPC3 antibody at -20˚ C. As with any antibody avoid repeat freeze-thaw cycles.

• Calculated Molecular Weight:

  64 kDa

• Applications Notes:

  GPC3 antibody can be used for detection of GPC3 by ELISA at 1:1562500. GPC3 antibody can be used for detection of GPC3 by western blot at 2.5 μ g/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.

• Symbol:

  GPC3

• NCBI Official Name:

  Glypican 3

• NCBI Organism:

  Homo sapiens

• Background Reference 01:

  Boily, G., et al., (2004) Br. J. Cancer 90 (8), 1606-1611.

• Other Product Names:

  GPC3, SGB, DGSX, MXR7, SDYS, SGBS, OCI-5, SGBS1, GTR2-2

• Tested Applications:

  ELISA, WB

• Protein ID:

  4758462

• Physical Properties:

  Liquid