PDLIM5 Antibody

• Background:

  PDLIM5 is a LIM domain protein. LIM domains are cysteine-rich double zinc fingers composed of 50 to 60 amino acids that are involved in protein-protein interactions. LIM domain-containing proteins are scaffolds for the formation of multiprotein complexes. The proteins are involved in cytoskeleton organization, cell lineage specification, organ development, and oncogenesis. The encoded protein is also a member of the Enigma class of proteins, a family of proteins that possess a 100-amino acid PDZ domain in the N terminus and 1 to 3 LIM domains in the C terminus. Multiple transcript variants encoding different isoforms have been found for this gene, although not all of them have been fully characterized.

• NCBI Gene ID:

  10611

• Swiss Prot:

  Q96HC4

• Accession Number:

  NP_001011513

• Host:

  Rabbit

• Reactivity:

  Human

• Immunogen:

  Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of human PDLIM5.

• Clonality:

  Polyclonal

• Conjugation:

  Unconjugated

• Type:

  Primary Antibodies

• Field of Research:

  Transcription

• Purification:

  Antibody is purified by protein A chromatography method.

• Positive Control:

  Cat. No. 1211 - HepG2 Cell Lysate

• Concentration:

  Batch dependent

• Buffer:

  Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose.

• Modification:

  None

• Shipping Conditions:

  Blue Ice

• Storage Conditions:

  For short periods of storage (days) store at 4˚ C. For longer periods of storage, store PDLIM5 antibody at -20˚ C. As with any antibody avoid repeat freeze-thaw cycles.

• Calculated Molecular Weight:

  54 kDa, 26 kDa, 24 kDa, 64 kDa

• Applications Notes:

  PDLIM5 antibody can be used for detection of PDLIM5 by ELISA at 1:312500. PDLIM5 antibody can be used for detection of PDLIM5 by western blot at 1.25 μ g/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.

• Symbol:

  PDLIM5

• NCBI Official Name:

  PDZ and LIM domain 5

• NCBI Organism:

  Homo sapiens

• Background Reference 01:

  Lasorella, A., et al., (2006) Proc. Natl. Acad. Sci. U.S.A. 103 (13), 4976-4981.

• Other Product Names:

  PDLIM5, L9, ENH, LIM, ENH1

• Tested Applications:

  ELISA, WB

• Protein ID:

  374093201

• Physical Properties:

  Liquid