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POU4F3 Antibody

CAT: 0223-27-780Size: 100 µLDry Ice: NoHazardous: No
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CAT#:0223-27-780Size:100 µL
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Background
POU4F3 is capable of activating both BDNF and NT-3 promoters in inner ear sensory epithelial cell lines. Mutant POU4F3 loses most of its transcriptional activity and most of its ability to bind to DNA. The mutation causes autosomal-dominant nonsyndromic hearing loss and eventually leads to hair cell morbidity in affected family members.
NCBI Gene ID
5459
Swiss Prot
Q15319
Accession Number
NP_002691
Host
Rabbit
Reactivity
Human, Mouse, Rat, Dog, Zebrafish
Immunogen
Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of human POU4F3.
Clonality
Polyclonal
Conjugation
Unconjugated
Type
Primary Antibodies
Field of Research
Transcription, Neuroscience
Purification
Antibody is purified by protein A chromatography method.
Positive Control
Cat. No. 1211 - HepG2 Cell Lysate
Concentration
Batch dependent
Buffer
Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose.
Modification
None
Shipping Conditions
Blue Ice
Storage Conditions
For short periods of storage (days) store at 4˚ C. For longer periods of storage, store POU4F3 antibody at -20˚ C. As with any antibody avoid repeat freeze-thaw cycles.
Calculated Molecular Weight
37 kDa
Applications Notes
POU4F3 antibody can be used for detection of POU4F3 by ELISA at 1:312500. POU4F3 antibody can be used for detection of POU4F3 by western blot at 1.25 μ g/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.
Symbol
POU4F3
NCBI Official Name
POU class 4 homeobox 3
NCBI Organism
Homo sapiens
Background Reference 01
Weiss, S., et al., (2003) Mol. Cell. Biol. 23 (22), 7957-7964.
Other Product Names
POU4F3, BRN3C, DFNA15
Tested Applications
ELISA, WB, IHC
Protein ID
4505965
Physical Properties
Liquid

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