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POU3F3 Antibody

CAT: 0223-57-077Size: 400 µLDry Ice: NoHazardous: No
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CAT#:0223-57-077Size:400 µL
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Background
POU3F3 is a member of the class III POU family of transcription factors (see POU3F1; MIM 602479) that are expressed in the central nervous system. The POU domain in these proteins is required for high affinity binding to octamer DNA sequences (summarized by Sumiyama et al., 1996 [PubMed 8703082]) .[supplied by OMIM].
NCBI Gene ID
5455
Swiss Prot
P20264
Accession Number
P20264
Host
Rabbit
Reactivity
Human
Immunogen
This POU3F3 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 294-323 amino acids from the Central region of human POU3F3.
Clonality
Polyclonal
Conjugation
Unconjugated
Type
Primary Antibodies
Field of Research
Neuroscience
Purification
This antibody is purified through a protein A column, followed by peptide affinity purification.
Concentration
Batch dependent
Homology
Predicted species reactivity based on immunogen sequence: Zebrafish, Mouse, Rat
Buffer
Supplied in PBS with 0.09% (W/V) sodium azide.
Modification
None
Shipping Conditions
Blue Ice
Storage Conditions
Store at 4˚ C for three months and -20˚ C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Calculated Molecular Weight
50 kDa
Fragment
Rabbit Ig
Applications Notes
For WB starting dilution is: 1:1000
Symbol
POU3F3
NCBI Official Name
POU domain, class 3, transcription factor 3
NCBI Organism
Homo sapiens
Background Reference 01
Wissmuller, S., et al. Nucleic Acids Res. 34 (6) :1735-1744 (2006)
Background Reference 02
Kuhlbrodt, K., et al. J. Biol. Chem. 273 (26) :16050-16057 (1998)
Background Reference 03
Sumiyama, K., et al. Mamm. Genome 9 (2) :180-181 (1998)
Background Reference 04
Malik, K.F., et al. Brain Res. Mol. Brain Res. 45 (1) :99-107 (1997)
Other Product Names
POU domain, class 3, transcription factor 3, Brain-specific homeobox/POU domain protein 1, Brain-1, Brn-1, Octamer-binding protein 8, Oct-8, Octamer-binding transcription factor 8, OTF-8, POU3F3, BRN1, OTF8
Tested Applications
WB
Protein ID
2506534
Physical Properties
Liquid

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