POU3F3 Antibody

• Background:

  POU3F3 is a member of the class III POU family of transcription factors (see POU3F1; MIM 602479) that are expressed in the central nervous system. The POU domain in these proteins is required for high affinity binding to octamer DNA sequences (summarized by Sumiyama et al., 1996 [PubMed 8703082]) .[supplied by OMIM].

• NCBI Gene ID:

  5455

• Swiss Prot:

  P20264

• Accession Number:

  P20264

• Host:

  Rabbit

• Reactivity:

  Human

• Immunogen:

  This POU3F3 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 294-323 amino acids from the Central region of human POU3F3.

• Clonality:

  Polyclonal

• Conjugation:

  Unconjugated

• Type:

  Primary Antibodies

• Field of Research:

  Neuroscience

• Purification:

  This antibody is purified through a protein A column, followed by peptide affinity purification.

• Concentration:

  Batch dependent

• Homology:

  Predicted species reactivity based on immunogen sequence: Zebrafish, Mouse, Rat

• Buffer:

  Supplied in PBS with 0.09% (W/V) sodium azide.

• Modification:

  None

• Shipping Conditions:

  Blue Ice

• Storage Conditions:

  Store at 4˚ C for three months and -20˚ C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

• Calculated Molecular Weight:

  50 kDa

• Fragment:

  Rabbit Ig

• Applications Notes:

  For WB starting dilution is: 1:1000

• Symbol:

  POU3F3

• NCBI Official Name:

  POU domain, class 3, transcription factor 3

• NCBI Organism:

  Homo sapiens

• Background Reference 01:

  Wissmuller, S., et al. Nucleic Acids Res. 34 (6) :1735-1744 (2006)

• Background Reference 02:

  Kuhlbrodt, K., et al. J. Biol. Chem. 273 (26) :16050-16057 (1998)

• Background Reference 03:

  Sumiyama, K., et al. Mamm. Genome 9 (2) :180-181 (1998)

• Background Reference 04:

  Malik, K.F., et al. Brain Res. Mol. Brain Res. 45 (1) :99-107 (1997)

• Other Product Names:

  POU domain, class 3, transcription factor 3, Brain-specific homeobox/POU domain protein 1, Brain-1, Brn-1, Octamer-binding protein 8, Oct-8, Octamer-binding transcription factor 8, OTF-8, POU3F3, BRN1, OTF8

• Tested Applications:

  WB

• Protein ID:

  2506534

• Physical Properties:

  Liquid