ELF3 Antibody

• Background:

  ELF3 is a novel, highly tissue-restricted member of the ets transcription factor/oncogene family. ELF3 contains two putative DNA binding domains: an ETS domain, which is unique in that the 5' half shows relatively weak homology to known ets factors, and an A/T hook domain, found in HMG proteins and various other nuclear factors. In contrast to any known ets factors, ELF3 is expressed exclusively in epithelial cells. ELF3 is expected to be a critical regulator of epithelial cell differentiation. ETS factor ELF3 is a transcriptional regulator of angiopoietin-1 gene regulation in the setting of inflammation. ELF3 overexpressed early during human breast tumorigenesis. ELF3 expression confers a transformed and in vitro metastatic phenotype to otherwise normal MCF-12A cells. ELF3 mediates the expression of TGF-beta RII, and the transcriptional inhibition of ets-related transcription factor could be a one of the mechanisms of colonic carcinogenesis. ELF3 is positively and negatively modulated by other interacting proteins including Ku70, Ku86, p300, and CBP.

• NCBI Gene ID:

  1999

• Swiss Prot:

  P78545

• Accession Number:

  NP_004424

• Host:

  Rabbit

• Reactivity:

  Human, Rat

• Immunogen:

  Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of human ELF3.

• Clonality:

  Polyclonal

• Conjugation:

  Unconjugated

• Type:

  Primary Antibodies

• Field of Research:

  Transcription

• Purification:

  Antibody is purified by protein A chromatography method.

• Positive Control:

  Cat. No. XBL-10426 - Fetal Thymus Tissue Lysate

• Concentration:

  Batch dependent

• Buffer:

  Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose.

• Modification:

  None

• Shipping Conditions:

  Blue Ice

• Storage Conditions:

  For short periods of storage (days) store at 4˚ C. For longer periods of storage, store ELF3 antibody at -20˚ C. As with any antibody avoid repeat freeze-thaw cycles.

• Calculated Molecular Weight:

  41 kDa

• Applications Notes:

  ELF3 antibody can be used for detection of ELF3 by ELISA at 1:312500. ELF3 antibody can be used for detection of ELF3 by western blot at 2.5 μ g/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.

• Symbol:

  ELF3

• NCBI Official Name:

  E74-like factor 3 (ets domain transcription factor, epithelial-specific)

• NCBI Organism:

  Homo sapiens

• Background Reference 01:

  Wang, H., (2004) J. Biol. Chem. 279 (24), 25241-25250.

• Other Product Names:

  ELF3, ERT, ESX, EPR-1, ESE-1

• Tested Applications:

  ELISA, WB

• Protein ID:

  167235023

• Physical Properties:

  Liquid