GALNT4 Antibody

GALNT4 catalyzes the initial reaction in O-linked oligosaccharide biosynthesis, the transfer of an N-acetyl-D-galactosamine residue to a serine or threonine residue on the protein receptor. It has a highest activity toward Muc7, EA2 and Muc2, with a lowest activity than GALNT2. This gene encodes a member of the UDP-N-acetyl-alpha-D-galactosamine:polypeptide N-acetylgalactosaminyltransferase (GalNAc-T) family of enzymes. GalNAc-Ts initiate mucin-type O-linked glycosylation in the Golgi apparatus by catalyzing the transfer of GalNAc to serine and threonine residues on target proteins. They are characterized by an N-terminal transmembrane domain, a stem region, a lumenal catalytic domain containing a GT1 motif and Gal/GalNAc transferase motif, and a C-terminal ricin/lectin-like domain. GalNAc-Ts have different, but overlapping, substrate specificities and patterns of expression. In vitro, the encoded protein can complement other GalNAc-Ts in the complete O-glycosylation of the mucin-1 tandem repeat and can O-glycosylate the P-selectin glycoprotein ligand-1 molecule. The coding region of this gene is contained within a single exon.

8693

Q8N4A0

NP_003765

Human, Mouse, Rat

Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of human GALNT4.

Unconjugated

Primary Antibodies

Membrane, Signal Transduction

Antibody is purified by peptide affinity chromatography method.

Cat. No. XBL-10123 - Fetal Brain Tissue Lysate

Batch dependent

Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose.

None

Blue Ice

For short periods of storage (days) store at 4˚ C. For longer periods of storage, store GALNT4 antibody at -20˚ C. As with any antibody avoid repeat freeze-thaw cycles.

67 kDa

GALNT4 antibody can be used for detection of GALNT4 by ELISA at 1:12500. GALNT4 antibody can be used for detection of GALNT4 by western blot at 1 μ g/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.

GALNT4

UDP-N-acetyl-alpha-D-galactosamine:polypeptide N-acetylgalactosaminyltransferase 4 (GalNAc-T4)

Homo sapiens

Lampasona, V., (er) J. Thromb. Thrombolysis (2008) In press.

GALNT4, GALNAC-T4, GalNAcT4, GALNACT4

ELISA, WB

34452725