CPT1A Antibody

• Background :

  The mitochondrial oxidation of long-chain fatty acids is initiated by the sequential action of carnitine palmitoyltransferase I (which is located in the outer membrane and is detergent-labile) and carnitine palmitoyltransferase II (which is located in the inner membrane and is detergent-stable), together with a carnitine-acylcarnitine translocase. CPT I is the key enzyme in the carnitine-dependent transport across the mitochondrial inner membrane and its deficiency results in a decreased rate of fatty acid beta-oxidation.The mitochondrial oxidation of long-chain fatty acids is initiated by the sequential action of carnitine palmitoyltransferase I (which is located in the outer membrane and is detergent-labile) and carnitine palmitoyltransferase II (which is located in the inner membrane and is detergent-stable), together with a carnitine-acylcarnitine translocase. CPT I is the key enzyme in the carnitine-dependent transport across the mitochondrial inner membrane and its deficiency results in a decreased rate of fatty acid beta-oxidation. Alternatively spliced transcript variants encoding different isoforms have been found for this gene.

• NCBI Gene ID :

  1374

• Swiss Prot :

  P50416

• Accession Number :

  NP_001027017

• Host :

  Rabbit

• Reactivity :

  Human

• Immunogen :

  Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of human CPT1A.

• Clonality :

  Polyclonal

• Conjugation :

  Unconjugated

• Type :

  Primary Antibodies

• Field of Research :

  Membrane

• Purification :

  Antibody is purified by peptide affinity chromatography method.

• Positive Control :

  293T Cell Lysate

• Concentration :

  Batch dependent

• Buffer :

  Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose.

• Modification :

  None

• Shipping Conditions :

  Blue Ice

• Storage Conditions :

  For short periods of storage (days) store at 4˚ C. For longer periods of storage, store CPT1A antibody at -20˚ C. As with any antibody avoid repeat freeze-thaw cycles.

• Calculated Molecular Weight :

  86 kDa

• Applications Notes :

  CPT1A antibody can be used for detection of CPT1A by ELISA at 1:312500. CPT1A antibody can be used for detection of CPT1A by western blot at 1 μ g/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.

• Symbol :

  CPT1A

• NCBI Official Name :

  Carnitine palmitoyltransferase 1A (liver)

• NCBI Organism :

  Homo sapiens

• Background Reference 01 :

  Roomets, E., (2008) Invest. Ophthalmol. Vis. Sci. 49 (4), 1660-1664.

• Other Product Names :

  CPT1A, CPT1, CPT1-L, L-CPT1

• Tested Applications :

  ELISA, WB

• Protein ID :

  73623028

• Physical Properties :

  Liquid