MSLN Antibody

• Background :

  An antibody that reacts with ovarian cancers and mesotheliomas was used to isolate a cell surface antigen named mesothelin. Although the function of mesothelin is unknown, it may play a role in cellular adhesion and is present on mesothelium, mesotheliomas, and ovarian cancers. This gene encodes a preproprotein that is cleaved into two products, megakaryocyte potentiating factor and mesothelin.An antibody that reacts with ovarian cancers and mesotheliomas was used to isolate a cell surface antigen named mesothelin. Although the function of mesothelin is unknown, it may play a role in cellular adhesion and is present on mesothelium, mesotheliomas, and ovarian cancers. This gene encodes a preproprotein that is cleaved into two products, megakaryocyte potentiating factor and mesothelin. Alternative splicing results in multiple transcript variants.

• NCBI Gene ID :

  10232

• Swiss Prot :

  Q13421-3

• Accession Number :

  NP_005814

• Host :

  Rabbit

• Reactivity :

  Human, Mouse

• Immunogen :

  Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of human MSLN.

• Clonality :

  Polyclonal

• Conjugation :

  Unconjugated

• Type :

  Primary Antibodies

• Field of Research :

  Membrane, Cancer, Signal Transduction

• Purification :

  Antibody is purified by peptide affinity chromatography method.

• Positive Control :

  Cat. No. 1219 - MCF7 Cell Lysate

• Concentration :

  Batch dependent

• Buffer :

  Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose.

• Modification :

  None

• Shipping Conditions :

  Blue Ice

• Storage Conditions :

  For short periods of storage (days) store at 4˚ C. For longer periods of storage, store MSLN antibody at -20˚ C. As with any antibody avoid repeat freeze-thaw cycles.

• Calculated Molecular Weight :

  32 kDa

• Applications Notes :

  MSLN antibody can be used for detection of MSLN by ELISA at 1:312500. MSLN antibody can be used for detection of MSLN by western blot at 1 μ g/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.

• Symbol :

  MSLN

• NCBI Official Name :

  Mesothelin

• NCBI Organism :

  Homo sapiens

• Background Reference 01 :

  Johnson, M.D., (2008) J. Histochem. Cytochem. 56 (6), 579-585.

• Other Product Names :

  MSLN, CAK1, MPF, SMR, SMRP

• Tested Applications :

  ELISA, WB

• Protein ID :

  53988378

• Physical Properties :

  Liquid