MSLN Antibody

• Background:

  An antibody that reacts with ovarian cancers and mesotheliomas was used to isolate a cell surface antigen named mesothelin. Although the function of mesothelin is unknown, it may play a role in cellular adhesion and is present on mesothelium, mesotheliomas, and ovarian cancers. This gene encodes a preproprotein that is cleaved into two products, megakaryocyte potentiating factor and mesothelin.An antibody that reacts with ovarian cancers and mesotheliomas was used to isolate a cell surface antigen named mesothelin. Although the function of mesothelin is unknown, it may play a role in cellular adhesion and is present on mesothelium, mesotheliomas, and ovarian cancers. This gene encodes a preproprotein that is cleaved into two products, megakaryocyte potentiating factor and mesothelin. Alternative splicing results in multiple transcript variants.

• NCBI Gene ID:

  10232

• Swiss Prot:

  Q13421-3

• Accession Number:

  NP_005814

• Host:

  Rabbit

• Reactivity:

  Human, Mouse

• Immunogen:

  Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of human MSLN.

• Clonality:

  Polyclonal

• Conjugation:

  Unconjugated

• Type:

  Primary Antibodies

• Field of Research:

  Membrane, Cancer, Signal Transduction

• Purification:

  Antibody is purified by peptide affinity chromatography method.

• Positive Control:

  Cat. No. 1219 - MCF7 Cell Lysate

• Concentration:

  Batch dependent

• Buffer:

  Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose.

• Modification:

  None

• Shipping Conditions:

  Blue Ice

• Storage Conditions:

  For short periods of storage (days) store at 4˚ C. For longer periods of storage, store MSLN antibody at -20˚ C. As with any antibody avoid repeat freeze-thaw cycles.

• Calculated Molecular Weight:

  32 kDa

• Applications Notes:

  MSLN antibody can be used for detection of MSLN by ELISA at 1:312500. MSLN antibody can be used for detection of MSLN by western blot at 1 μ g/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.

• Symbol:

  MSLN

• NCBI Official Name:

  Mesothelin

• NCBI Organism:

  Homo sapiens

• Background Reference 01:

  Johnson, M.D., (2008) J. Histochem. Cytochem. 56 (6), 579-585.

• Other Product Names:

  MSLN, CAK1, MPF, SMR, SMRP

• Tested Applications:

  ELISA, WB

• Protein ID:

  53988378

• Physical Properties:

  Liquid