CD86 Antibody

• Background:

  CD86, also known as B7-2, is a type I membrane protein that is a member of the immunoglobulin superfamily. Like the related protein CD80, this protein is expressed by antigen-presenting cells, and is the ligand for two proteins at the cell surface of T cells, CD28 and the cytotoxic T-lymphocyte-associated protein 4 (CTLA-4) . Binding of this protein with CD28 antigen is a costimulatory signal for activation of the T-cell and induces T-cell proliferation and cytokine production. CTLA-4 binding negatively regulates T-cell activation and diminishes the immune response (1) . Blocking the CTLA-4-CD80/CD86 interaction has been shown to enhance T-cell functions in acute lymphoblastomic leukemia (ALL), suggesting that this pathway may be an attractive target for future cancer immunotherapy (2) .

• NCBI Gene ID:

  942

• Swiss Prot:

  P42082

• Accession Number:

  NP_787058

• Host:

  Rabbit

• Reactivity:

  Human

• Clonality:

  Polyclonal

• Conjugation:

  Unconjugated

• Type:

  Primary Antibodies

• Field of Research:

  Cell Cycle, Cancer, Immunology

• Purification:

  CD86 Antibody is affinity chromatography purified via peptide column.

• Positive Control:

  Cat. No. 1224 - Daudi Cell Lysate

• Concentration:

  1 mg/mL

• Homology:

  Predicted species reactivity based on immunogen sequence: Rabbit: (75%), Mouse: (75%)

• Buffer:

  CD86 Antibody is supplied in PBS containing 0.02% sodium azide.

• Modification:

  None

• Shipping Conditions:

  Blue Ice

• Storage Conditions:

  CD86 antibody can be stored at 4˚ C for three months and -20˚ C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

• Fragment:

  IgG

• Specificity:

  At least five isoforms of CD86 are known to exist; this antibody will detect all five isoforms.

• Symbol:

  CD86

• Positive Control 2:

  Cat. No. 1207 - Raji Cell Lysate

• NCBI Official Name:

  CD86 molecule

• NCBI Organism:

  Homo sapiens

• Background Reference 01:

  Lane P. Regulation of T and B cell responses by modulating interactions between CD28/CTLA-4 and their ligands, CD80 and CD86. Ann NY Acad Sci 1997; 815:392-400.

• Background Reference 02:

  Feucht J, Kayser S, Gorodezki D, et al. T-cell responses against CD19+ pediatric acute lymphoblastic leukemia mediated by bispecific T-cell engager (BiTE) are regulated contrarily by PD-L1 and CD80/CD86 on leukemic blasts. Oncotarget 2016; 7:76902-19.

• Other Product Names:

  CD86 Antibody: CD86 molecule, B70, B7-2, B7.2, LAB72, CD28LG2

• Tested Applications:

  ELISA, WB, IHC-P, IF

• Protein ID:

  332634934

• Physical Properties:

  Liquid