TIM-3 Antibody

• Background:

  The immune checkpoint protein TIM-3 is a member of the immunoglobulin superfamily and TIM family of proteins that was initially identified as a specific marker of fully differentiated IFN-γ producing CD4 T helper 1 (Th1) and CD8 cytotoxic cells. It is a Th1-specific cell surface protein that regulates macrophage activation and negatively regulates Th1-mediated auto- and alloimmune responses, and is also highly expressed on regulatory T cells, monocytes, macrophages, and dendritic cells (1) . TIM-3 and PD-1 are co-expressed on most CD4 and CD8 T cells infiltrating solid tumors or in hematologic malignancy in mice; blocking TIM-3 in conjugation with a PD-1 blockade increases the functionality of exhausted T cells and synergizes with to inhibit tumor growth (2,3) .

• NCBI Gene ID:

  84868

• Swiss Prot:

  Q8TDQ0

• Accession Number:

  NP_116171

• Host:

  Rabbit

• Reactivity:

  Human

• Clonality:

  Polyclonal

• Conjugation:

  Unconjugated

• Type:

  Primary Antibodies

• Field of Research:

  Cell Cycle, Cancer, Immunology

• Purification:

  TIM-3 Antibody is affinity chromatography purified via peptide column.

• Positive Control:

  Cat. No. 1205 - Jurkat Cell Lysate

• Concentration:

  1 mg/mL

• Buffer:

  TIM-3 Antibody is supplied in PBS containing 0.02% sodium azide.

• Modification:

  None

• Shipping Conditions:

  Blue Ice

• Storage Conditions:

  TIM-3 antibody can be stored at 4˚ C for three months and -20˚ C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

• Fragment:

  IgG

• Specificity:

  At least two antibodies are known to exist; this antibody will detect both isoforms.

• Symbol:

  HAVCR2

• NCBI Official Name:

  Hepatitis A virus cellular receptor 2

• NCBI Organism:

  Homo sapiens

• Background Reference 01:

  Monney L, Sabatos CA, Gaglia JL, et al. Th1-specific cell surface protein Tim-3 regulates macrophage activation and severity of autoimmune disease. Nature 2002; 415:536-41.

• Background Reference 02:

  Sakuishi K, Apetoh L, Sullivan JM, et al. Targeting Tim-3 and PD-1 pathways to reverse T cell exhaustion and restore anti-tumor immunity. J Exp Med 2010; 207:2187–94.

• Background Reference 03:

  Zhou Q, Munger ME, Veenstra RG, et al. Coexpression of Tim-3 and PD-1 identifies a CD8+ T-cell exhaustion phenotype in mice with disseminated acute myelogenous leukemia. Blood 2011; 117:4501–10.

• Other Product Names:

  TIM-3 Antibody: Hepatitis A virus cellular receptor, HAVCR2, TIM-3, CD366, KIM-3, TIMD3, TIMD-3

• Tested Applications:

  ELISA, WB, IHC-P, IF

• Protein ID:

  84868

• Physical Properties:

  Liquid