CRBN Antibody

• Background :

  CRBN, a member of the Lon protease protein family, plays a role in brain development (1) . It is a component of the DCX (DDB1-CUL4-X-box) E3 protein ligase complex, a complex that mediates the ubiquitination and subsequent proteasomal degradation of target proteins and is required for limb outgrowth and expression of the fibroblast growth factor FGF8 (2) . CRBN is thought to regulate the assembly and neuronal surface expression of large-conductance calcium-activated potassium channels in brain regions involved in memory and learning via its interaction with KCNT1. It is widely expressed and highly expressed in brain (3,4) .

• NCBI Gene ID :

  51185

• Swiss Prot :

  Q96SW2

• Accession Number :

  NP_057386

• Host :

  Rabbit

• Reactivity :

  Human, Mouse, Rat

• Clonality :

  Polyclonal

• Conjugation :

  Unconjugated

• Type :

  Primary Antibodies

• Field of Research :

  Neuroscience, Stem Cell

• Purification :

  CRBN antibody is affinity chromatography purified via peptide column.

• Positive Control :

  Cat. No. 1313 - Human Testis Tissue Lysate

• Concentration :

  1 mg/mL

• Homology :

  Predicted species reactivity based on immunogen sequence: Bovine: (94%)

• Buffer :

  CRBN antibody is supplied in PBS containing 0.02% sodium azide.

• Modification :

  None

• Shipping Conditions :

  Blue Ice

• Storage Conditions :

  CRBN antibody can be stored at 4˚ C up to one year. Antibodies should not be exposed to prolonged high temperatures.

• Fragment :

  IgG

• Specificity :

  CRBN antibody is human, mouse and rat reactive. At least two isoforms of CRBN are known to exist; this antibody will detect both isoforms.

• Symbol :

  CRBN

• NCBI Official Name :

  Cereblon

• NCBI Organism :

  Homo sapiens

• Background Reference 01 :

  Xin W, Xiaohua N, Peilin C, et al. Primary function analysis of human mental retardation related gene CRBN. Mol. Biol. Rep. 2008; 35:251-6.

• Background Reference 02 :

  Gandhi AK, Kang J, Havens CG, et al. Immunomodulatory agents lenalidomide and pomalidomide co-stimulate T cells by inducing degradation of T cell repressors Ikaros and Aiolos via modulation of the E3 ubiquitin ligase complex CRL4 (CRBN) . Br. J. Haematol. 2014; 164:811-21.

• Background Reference 03 :

  Liu J, Ye J, Zou X, et al. CRL4A (CRBN) E3 ubiquitin ligase restricts BK channel activity and prevents epileptogenesis. Nat. Commun. 2014; 5:3924.

• Background Reference 04 :

  Heintel D, Rocci A, Ludwig H, et al. High expression of cereblon (CRBN) is associated with improved clinical response in patients with multiple myeloma treated with lenalidomide and dexamethasone. Br. J. Haematol. 2013; 161:695-700.

• Other Product Names :

  Cereblon, AD-006, DKFZp781K0715, MGC27358, MRT2A

• Tested Applications :

  ELISA, WB, IHC-P, IF

• Protein ID :

  39545580

• Physical Properties :

  Liquid