E2F3 Antibody

• Background:

  The E2F transcription factor 3 (E2F3) is a member of a small family of transcription factors that function through binding of DP interaction partner proteins. E2F3 recognizes a specific sequence motif in DNA and interacts directly with the retinoblastoma protein (pRB) to regulate the expression of genes involved in the cell cycle (1) . Like the related E2F1 and E2F2, E2F3 is essential for cellular proliferation and progression through the cell cycle (2) . Altered copy number and activity of this gene have been observed in a number of human cancers (3) .

• NCBI Gene ID:

  1871

• Swiss Prot:

  O00716

• Accession Number:

  NP_001940

• Host:

  Rabbit

• Reactivity:

  Human, Mouse

• Clonality:

  Polyclonal

• Conjugation:

  Unconjugated

• Type:

  Primary Antibodies

• Field of Research:

  Cancer, Cell Cycle

• Purification:

  E2F3 antibody is affinity chromatography purified via peptide column.

• Positive Control:

  Cat. No. 1369 - Human Lymph node Tissue Lysate

• Concentration:

  1 mg/mL

• Buffer:

  E2F3 antibody is supplied in PBS containing 0.02% sodium azide.

• Modification:

  None

• Shipping Conditions:

  Blue Ice

• Storage Conditions:

  E2F3 antibody can be stored at 4˚ C for three months and -20˚ C, stable for up to one year.

• Fragment:

  IgG

• Specificity:

  E2F3 antibody is human and mouse reactive. At least three isoforms of E2F3 are known to exist; this antibody will detect only the largest isoform. This antibody is predicted to not cross-react with other members of the E2F transcription factor family.

• Symbol:

  E2F3

• Positive Control 2:

  Cat. No. 11-521 - Human Lymphoid Tissue Tissue Slide

• NCBI Official Name:

  E2F transcription factor 3

• NCBI Organism:

  Homo sapiens

• Background Reference 01:

  Lees JA, Saito M, Vidal M, et al. The retinoblastoma protein binds to a family of E2F transcription factors. Mol. Cell. Biol. 1993; 13:7813-25.

• Background Reference 02:

  Wu L, Timmers C, Maiti B, et al. The E2F1-3 transcription factors are essential for cellular proliferation. Nature 2001; 414:457-62.

• Background Reference 03:

  DeGregori J. The genetics of the E2F family of transcription factors: shared functions and unique roles. Biochim. Biophys. Acta. 2002; 1602:131-50.

• Other Product Names:

  E2F transcription factor 3, E2F-3

• Tested Applications:

  ELISA, WB, IHC-P, IF

• Protein ID:

  4503433

• Physical Properties:

  Liquid